APPLICATION NOTE
Results The M1050 Lyophilized NanoAdapter Kit demonstrated sequencing performance comparable to the validated liquid M1050 formulation across all evaluated 4bases library types. Representative results from each library type are shown in Figures 3–5. For both short- and long-amplicon libraries, sequencing yield, read count and fragment size distribution (N50) were highly consistent between the two NanoAdapter formulations. Similar pore occupancy profiles and Q score distributions further confirmed that the lyophilized format maintains compatibility with the Oxford Nanopore sequencing chemistry. Comparable results were also obtained for capture-based libraries, supporting the use of the lyophilized M1050 NanoAdapter Kit as a standardized final step for Oxford Nanopore compatibility across different 4bases library preparation strategies.
Figure 3. Representative sequencing results from a short-amplicon 4bases library processed with either the liquid M1050 NanoAdapter Kit or the M1050 Lyophilized NanoAdapter Kit.
Figure 4. Representative sequencing results from a long-amplicon 4bases library processed with either the liquid M1050 NanoAdapter Kit or the M1050 Lyophilized NanoAdapter Kit.
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