APPLICATION NOTE
Across all tested applications, the lyophilized formulation maintained sequencing quality, pore utilization and overall workflow performance without requiring changes to the upstream 4bases library preparation protocols.
Figure 5. Representative sequencing results from a capture-based 4bases library processed with either the liquid M1050 NanoAdapter Kit or the M1050 Lyophilized NanoAdapter Kit.
Conclusion The M1050 NanoAdapter Kit connects 4bases library preparation workflows with Oxford Nanopore sequencing. By converting 4bases-prepared libraries into a format compatible with the Ligation Adapter and motor protein chemistry of the Oxford Nanopore Technologies Ligation Sequencing Kit V14 (SQK-LSK114), NanoAdapter enables a streamlined transition to nanopore sequencing. The liquid M1050 formulation had already been tested and validated across short- amplicon, long-amplicon and capture-based applications. The new lyophilized formulation, developed through the combined R&D expertise of Meridian Life Science and 4bases, generated comparable sequencing yield, read count, fragment size distribution, pore occupancy and sequencing quality. These results support the integration of the M1050 Lyophilized NanoAdapter Kit into existing 4bases workflows as a reliable, lyophilized solution that strengthens Oxford Nanopore compatibility while simplifying reagent handling, storage and transportation.
Key Benefits of the Lyophilized Formulation
Simplified reagent handling Increased reproducibility among experiments Improved storage and transportation
Compatible with existing workflows Validated across multiple applications Comparable sequencing performance
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