More confidence when low-copy targets matter. Residual E. coli DNA associated with recombinant enzymes produced in bacterial systems can create background signal and increase false-positive risk. Meridian non-bacterial (Non-Bac) qPCR solutions use a eukaryotic expression system to support sensitive detection of low-copy bacterial targets.
Non-Bac DNA qPCR Solutions Cleaner background for sensitive bacterial DNA detection
Background bacterial DNA does not have to be an accepted limitation.
More confidence when low-copy targets matter Residual E. coli DNA associated with recombinant enzymes produced in bacterial systems can create background signal and increase false-positive risk. Meridian non-bacterial (Non-Bac) qPCR solutions use a eukaryotic expression system to support sensitive detection of low-copy bacterial targets. From development to commercialization Start with the format that suits your development workflow today, while retaining a path to stabilization later. Choose MDX252 for custom formulation or MDX362 for a complete Lyo-Ready qPCR mix.
Choose the format that matches your workflow
MDX252 Custom formulation Design control for custom PCR/qPCR development.
MDX362 Ready-to-use Complete 4x qPCR mix for ready-to-use simplicity.
MDX252
Lyo-Ready Non-Bac DNA qPCR Mix • Complete 4x mix: Hot-start qPCR mix for sensitive low-copy multiplex detection. • Non-Bac format: Designed to help reduce bacterial DNA background. • Liquid or lyophilized: Supports ambient-stable assay formats. MDX362 Glycerol-Free Non-Bac Taq HS Polymerase (HC) • H igh concentration: 50 U/ µ L hot-start Taq polymerase. • Custom formulation: Glycerol-free format for PCR and qPCR mix development. • Dry-format compatible: Supports lyophilized assay development with appropriate excipients.
Both support wet-format development today with a path to lyophilization later.
ORDERING INFORMATION Product CAT. No
Size
1 mL (200 reactions) 5 mL (1,000 reactions) 50 mL (10,000 reactions) 20 µ L (1,000 units) 500 µ L (25,000 units) 5 mL (250,000 units)
Lyo-Ready Non-Bac DNA qPCR Mix
MDX362
Glycerol-Free Non-Bac Taq HS Polymerase (HC)
MDX252
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Non-Bac DNA qPCR Solutions Cleaner background for sensitive bacterial DNA detection
MDX362 Performance Data
Designed for Sensitive Bacterial DNA Detection The completed 4x hot-start mix containing optimized buffer chemistry, PCR enhancers, dNTPs (including dUTP), MgCl 2 and Glycerol-Free Non-Bac HS Taq polymerase, supports low-copy and multiplex qPCR testing. It can be used as a liquid mix or lyophilized for ambient-stable assay formats.
10,000 copies 1,000 copies 100 copies 10 copies 1 copy
Lyo-Ready Non-Bac DNA qPCR Mix in liquid (blue) and lyophilized (red) format were compared for their ability to amplify a 10-fold serial dilution of bacterial DNA target (10,000, 1,000, 100, 10 and 1 copies respectively). The results illustrate that the lyophilized mix maintains the same high application efficiency as the liquid mix, with high sensitivity and reproducibility. NTCs are shown in (black) .
No Detectable Bacterial DNA Contamination
Using a pan-bacterial 16S assay, MDX362 showed high sensitivity and reproducibility while the no-template control remained without detectable amplification. Lyo-Ready Non-Bac DNA qPCR Mix was used to amplify a 10-fold serial dilution of bacterial DNA target (10,000, 1,000, 100, 10, and 1 copies, respectively) in liquid (blue) format using a pan-bacterial 16S assay. No detectable amplification was observed in the no-template control (black) . The Lyo-Ready Non-Bac DNA qPCR Mix demonstrates high
10,000 copies 1,000 copies 100 copies 10 copies 1 copy
sensitivity and reproducibility, supporting reliable detection of low-copy bacterial DNA targets with no detectable bacterial background amplification under the conditions tested.
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Non-Bac DNA qPCR Solutions Cleaner background for sensitive bacterial DNA detection
MDX362 Performance Data continued
High Multiplexing Capability P. aeruginosa
M. tuberculosis
E. coli
1,000 copies 100 copies 10 copies 1 copy
1,000 copies 100 copies 10 copies 1 copy
1,000 copies 100 copies 10 copies 1 copy
Three bacterial DNA targets were amplified using Lyo-Ready Non-Bac DNA qPCR Mix in liquid (blue) format, under standard qPCR cycling conditions. The results demonstrate the high sensitivity of Lyo-Ready Non-Bac DNA qPCR Mix, enabling reliable amplification of bacterial targets down to single-copy levels in multiplex reactions. NTCs are shown in (black) .
Lyophilized Assay Stability Lyo-Ready Non-Bac DNA qPCR Mix was lyophilized, and the stability of the dried assays (red) was tested after accelerated stability against fresh liquid mix (blue) in a triplex qPCR assay. The lyophilized mix retained performance comparable to the liquid mix, supporting projected stability of more than 12 months at ambient temperature.
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Non-Bac DNA qPCR Solutions Cleaner background for sensitive bacterial DNA detection
MDX362 Performance Data continued
High Multiplexing Capability 16S (Entero)
Mip
ToxA
1,000 copies 100 copies 10 copies 1 copy
1,000 copies 100 copies 10 copies 1 copy
1,000 copies 100 copies 10 copies 1 copy
The activity of the Glycerol-Free Non-Bac HS Taq Polymerase (HC) was tested in a triplex qPCR assay targeting marker genes from three bacteria (16S rRNA gene from Enterococcus , Mip gene from Legionella pneumophila and ToxA gene from Pseudomonas aeruginosa ), using a 10-fold serial dilution of the bacterial genomic DNA. The result shows that Glycerol-Free Non-Bac HS Taq Polymerase (HC) can successfully detect multiple targets down to 1 copy of DNA in multiplex assays.
Lyophilized Assay Stability Glycerol-Free Non-Bac HS Taq Polymerase (HC) was lyophilized in a lyo-ready DNA qPCR mix and the stability of the dried assay (red) was tested after accelerated stability against fresh liquid mix (blue) in a triplex qPCR assay. The lyophilized mixes retained the same performance as the liquid. The lyophilized mix retained performance comparable to the liquid mix, supporting projected stability of more than 12 months at ambient temperature.
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Email: info@meridianlifescience.com Orders: orders@meridianlifescience.com
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