Non-Bac DNA qPCR Solutions Cleaner background for sensitive bacterial DNA detection
MDX362 Performance Data continued
High Multiplexing Capability 16S (Entero)
Mip
ToxA
1,000 copies 100 copies 10 copies 1 copy
1,000 copies 100 copies 10 copies 1 copy
1,000 copies 100 copies 10 copies 1 copy
The activity of the Glycerol-Free Non-Bac HS Taq Polymerase (HC) was tested in a triplex qPCR assay targeting marker genes from three bacteria (16S rRNA gene from Enterococcus , Mip gene from Legionella pneumophila and ToxA gene from Pseudomonas aeruginosa ), using a 10-fold serial dilution of the bacterial genomic DNA. The result shows that Glycerol-Free Non-Bac HS Taq Polymerase (HC) can successfully detect multiple targets down to 1 copy of DNA in multiplex assays.
Lyophilized Assay Stability Glycerol-Free Non-Bac HS Taq Polymerase (HC) was lyophilized in a lyo-ready DNA qPCR mix and the stability of the dried assay (red) was tested after accelerated stability against fresh liquid mix (blue) in a triplex qPCR assay. The lyophilized mixes retained the same performance as the liquid. The lyophilized mix retained performance comparable to the liquid mix, supporting projected stability of more than 12 months at ambient temperature.
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411-13 MLS Non-Bac DNA qPCR Solutions MECH.indd 4
10/20/26 12:50 PM
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