Meridian Air Dryable Direct Urine

Superior reaction efficiency using crude urine samples in a multiplex reaction

Air-Dryable ™ Direct DNA qPCR Urine (MDX150)

Neisseria gonorrhoeae

Chlamydia trachomatis

GBS

Air-dried | KAPA Probe Force (Roche) | Ultraplex ™ (QuantBio)

Targets from Neisseria gonorrhoeae, Chlamydia trachomatis and group B streptococcus (GBS) were amplified in a triplex reaction in the presence of 25% human urine, using air-dried Air-Dryable ™ Direct DNA qPCR Urine ( red ) and KAPA Probe Force (Roche, green ) and Ultraplex ™ (QuantBio, grey ). The results illustrate higher multiplexing capacity and much better sensitivity using air-dried Air-Dryable Direct DNA qPCR Urine in the presence of 25% human urine.

Air-Dryable ™ Direct RNA/DNA qPCR Urine (MDX151)

Dengue

Zika

Chikungunya

Air-dried | KAPA Probe Force (Roche) | Ultraplex ™ (QuantBio)

Three viral RNA targets (Dengue, Zika and Chikungunya) were amplified in a triplex reaction using air-dried Air-Dryable ™ Direct RNA/DNA qPCR Urine ( red ) and kits from KAPA Probe Force (Roche, green ) and Ultraplex ™ (QuantBio, grey ) in the presence of 10% human urine. The results illustrate that dry Air-Dryable Direct RNA/DNA qPCR Urine has higher multiplexing capacity and reproducibility in the presence of 10% urine.

Mixes demonstrate high performance (sensitivity and reproducibility) in both liquid and dried-down formats

Air-dried | Wet Mix | Reliance (Bio-Rad)

A) Activity of Air-Dryable ™ Direct DNA qPCR Urine in air-dried ( red ) and liquid ( blue ) format was compared in the ability to amplify cell-surface opacity protein gene target from Neisseria gonorrhoeae (strain FA1090) genomic DNA using a 10-fold serial dilution of bacterial DNA (20,000, 2000, 200 and 20 copies respectively) in the presence of 30% pooled human urine in a multiplexing qPCR assay. B) Activity of Air-Dryable ™ Direct RNA/DNA qPCR Urine in air-dried ( red ) and wet ( blue ) format were compared for their ability to amplify a 10-fold RNA target (10,000, 1,000, 100 and 10 copies respectively) in the presence of 5% human urine. C) Activity of air-dried Air-Dryable ™ Direct RNA/DNA qPCR Urine ( red ) was compared to Bio-Rad (Reliance, purple ) for their ability to amplify a 1000-fold dilution of an RNA target (10,000 and 10 copies respectively) in the presence of 5% human urine. The results illustrate that the dried mixes maintain the same high performance as the liquid mixes and shows much higher sensitivity than Reliance 1-Step Multiplex RT-qPCR Supermix.