分子试剂 优化的RT-qPCR 预混液
MDX025 Low LOD一步法RT-qPCR预混液 适用于检测非常低水平的RNA和DNA病毒,如血库或移植病毒检测。为多重分析优化的新型缓冲系统,可在一根试管中进行第一步DNA合成 和qPCR。
产品性能数据: RNA和DNA病毒的低检测限(LOD)
Amplification Curves Amplification Curves HIV-2 (5 IU/mL)
Amplification Curves mplification Curves HCV (30 IU/mL) Amplification Curves
23.688 23.079 23.079 23.6 8
40.432
21.688 21.079 21.079 21.6 8
36.932
19.688 19.079 19.079 19.6 8
33.432
17.688 17.079 17.079 17.6 8
29.932
15.688 15.079 15.079 15.6 8
26.432
13.688 13.079 13.079 13.688
22.932
11.688 11.079 11.079 1.6 8
19.432
9.688 9.079 9.079 9.6 8
15.932
7.688 7.079 7.079 7.6 8
12.432
5.688 5.079 5.079 3.688 3.079 3.079 3.6 8 1.688 1.079 1.079 1.6 8 5.6 8
8.932
5.432
1.932
2 4 6 8 10 2 4 6 8 10 12
12 12
14 14 20 14
16 16
18 18 26
20 20
22 22
24 24 24
26 26
28 28
30 30
32 32 32
34 34 34
36 36 36
38 38 38
40 40 40
42 42 42
44 44 4
10 4 16
18
22
24
28
22 30 3
34
36
38
40
42
44
2
4
6
8
12
16
18
20
26
28
30
2
4
6
8
10
2
4
6
8
10
12
14
16
18
20
22
24
26
28
30
32
34
36
38
40
42
44
Cycles
Cycles
Cycles
Cycles
Cycles
Amplification Curves HAV (5 IU/mL) Amplification Curves
Amplification Curves HBV (15 IU/mL) Amplification Curves
23.688
6.628
21.688
6.028
19.688
5.428
17.688
4.828
15.688
4.228
13.688
3.628
11.688
3.028
9.688
2.428
7.688
1.828
5.688
1.228
3.688
0.628
1.688
0.028
2
4
6
8
10
12
14
16
18
20
22
24
26
28
30
32
34
36
38
40
42
44
2
4
6
8
10
12
14
16
18
20
22
24
26
28
30
32
34
36
38
40
42
44
Cycles
Cycles
Cycles
Cycles
Amplification Curves Amplification Curves B19 (60 IU/mL)
Amplification Curves Amplification Curves HIV-1 (60 IU/mL)
63.075
43.077
57.075
39.077
35.077
51.075
31.077
45.075
27.077
39.075
23.077
33.075
19.077
27.075
15.077
21.075
11.077
15.075
7.077
9.075
3.077
3.075
2
4
6
8
10
12
14
16
18
20
22
24
26
28
30
32
34
36
38
40
42
44
2
4
6
8
10
12
14
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20
22
24
26
28
30
32
34
36
38
40
42
44
Cycles
Cycles
Cycles
Cycles
对24份纯化血液病毒提取物的重复检测采用单倍qPCR反应,使用Low LOD 一步法RT-qPCR预混液在指示的IU/mL处进行。 单重反应为HAV、HBV、HCV、HIV-1、HIV-2、细小病毒B19。扩增曲线结果表明,Low LOD 一步法RT-qPCR预混液既可 用于RNA病毒,也可用于低至5IU/mL的DNA病毒。
测序级别核苷酸 即用型测序级别核苷酸,可单独提供,作为一套,或预先混合的混合液。超纯dNTPs作为锂盐供应。不含PCR抑制剂、DNase和RNase。 获取更多信息,请浏览官方网站: www.MeridianLifeScience.com.
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