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分子试剂 优化的RT-qPCR 预混液

MDX025 Low LOD一步法RT-qPCR预混液 适用于检测非常低水平的RNA和DNA病毒,如血库或移植病毒检测。为多重分析优化的新型缓冲系统,可在一根试管中进行第一步DNA合成 和qPCR。

产品性能数据: RNA和DNA病毒的低检测限(LOD)

Amplification Curves Amplification Curves HIV-2 (5 IU/mL)

Amplification Curves mplification Curves HCV (30 IU/mL) Amplification Curves

23.688 23.079 23.079 23.6 8

40.432

21.688 21.079 21.079 21.6 8

36.932

19.688 19.079 19.079 19.6 8

33.432

17.688 17.079 17.079 17.6 8

29.932

15.688 15.079 15.079 15.6 8

26.432

13.688 13.079 13.079 13.688

22.932

11.688 11.079 11.079 1.6 8

19.432

9.688 9.079 9.079 9.6 8

15.932

7.688 7.079 7.079 7.6 8

12.432

5.688 5.079 5.079 3.688 3.079 3.079 3.6 8 1.688 1.079 1.079 1.6 8 5.6 8

8.932

5.432

1.932

2 4 6 8 10 2 4 6 8 10 12

12 12

14 14 20 14

16 16

18 18 26

20 20

22 22

24 24 24

26 26

28 28

30 30

32 32 32

34 34 34

36 36 36

38 38 38

40 40 40

42 42 42

44 44 4

10 4 16

18

22

24

28

22 30 3

34

36

38

40

42

44

2

4

6

8

12

16

18

20

26

28

30

2

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10

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Cycles

Cycles

Cycles

Cycles

Cycles

Amplification Curves HAV (5 IU/mL) Amplification Curves

Amplification Curves HBV (15 IU/mL) Amplification Curves

23.688

6.628

21.688

6.028

19.688

5.428

17.688

4.828

15.688

4.228

13.688

3.628

11.688

3.028

9.688

2.428

7.688

1.828

5.688

1.228

3.688

0.628

1.688

0.028

2

4

6

8

10

12

14

16

18

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42

44

Cycles

Cycles

Cycles

Cycles

Amplification Curves Amplification Curves B19 (60 IU/mL)

Amplification Curves Amplification Curves HIV-1 (60 IU/mL)

63.075

43.077

57.075

39.077

35.077

51.075

31.077

45.075

27.077

39.075

23.077

33.075

19.077

27.075

15.077

21.075

11.077

15.075

7.077

9.075

3.077

3.075

2

4

6

8

10

12

14

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Cycles

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对24份纯化血液病毒提取物的重复检测采用单倍qPCR反应,使用Low LOD 一步法RT-qPCR预混液在指示的IU/mL处进行。 单重反应为HAV、HBV、HCV、HIV-1、HIV-2、细小病毒B19。扩增曲线结果表明,Low LOD 一步法RT-qPCR预混液既可 用于RNA病毒,也可用于低至5IU/mL的DNA病毒。

测序级别核苷酸 即用型测序级别核苷酸,可单独提供,作为一套,或预先混合的混合液。超纯dNTPs作为锂盐供应。不含PCR抑制剂、DNase和RNase。 获取更多信息,请浏览官方网站: www.MeridianLifeScience.com.

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