Saliva Specimen-specific Master Mixes for Multiplex Assays

Results: In comparison to Air-Dryable ™ 1-Step RT-qPCR Mix (MDX095), Air-Dryable ™ DIRECT RNA/DNA qPCR Saliva (MDX131) demonstrated faster Cts across all sample, for both standard and fast cycling conditions (Figure 2). This suggests that MDX131 has greater inhibitor tolerance to inhibitors found in saliva, including carry-over inhibitors still present in the extracted samples. Conclusion Air-Dryable ™ Direct RNA/DNA qPCR Saliva Mix (MDX131) and Air-Dryable ™ 1-Step RT-qPCR Mix (MDX095) were both able to perform better under fast cycling conditions, in contrast to a universal inhibitor tolerant reference mix, however Air-Dryable ™ Direct RNA/DNA qPCR Saliva Mix (MDX131) performed much better than Air-Dryable ™ 1-Step RT-qPCR Mix in the presence of both purified RNA from infected human saliva and RNA in crude infected human saliva lysate. The results show that universal inhibitor tolerant reference mix will work with saliva, but they are not as fast or as sensitive as a mix designed specifically for crude saliva lysates, making Air-Dryable ™ Direct RNA/DNA qPCR Saliva Mix ideal for SARS-CoV-2 and influenza viral targets, even after the mix has been dried-down and stored at ambient temperature.

References: 1. To, K.K., et. al. Additional molecular testing of saliva specimens improves the detection of respiratory viruses. Emerg Microbes Infect. 2017 Jun 7;6(6):e49. doi: 10.1038/ emi.2017.35. 2. Wang, C., et. al. A New Specimen for Syphilis Diagnosis: Evidence by High Loads of Treponema pallidum DNA in Saliva, Clinical Infectious Diseases . 2021 Nov 1; 73(9), e3250–e3258. doi:10.1093/cid/ciaa1613 3. Valinetz, E. and Cangelosi, G. A Look Inside: Oral Sampling for Detection of Non-oral Infectious Diseases. Journal of Clinical Microbiology . 2021;59(10). doi:10.1128/ JCM.02360-20 4. Oberkanins, C., et al. Use of saliva and salivary DNA for comprehensive genotyping based on RealFast and StripAssays. Ann Transl Med . 2017 Sep;5(Suppl 2):AB079. doi: 10.21037/atm.2017.s079.

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