Performance Data K-Block TM demonstrates superior HAMA and RF blocking over Mouse IgG and other commercial HAMA blockers.
A.
B.
Relative HAMA Blocking Capacity
Relative RF Blocking Capacity
100
100
K-Block TM
K-Block TM
Native mouse IgG
Native mouse IgG
80
80
Competitor Blocker #1 Competitor Blocker #2
Competitor Blocker #1 Competitor Blocker #2
60
60
40
40
20
20
0
0
0.0001
0.001
0.01 Blocker Concentration ( µ g/ml)
0.1
1
0.01
0.1
Blocker Concentration ( µ g/ml) 1
10
100
HAMA Blocking Strength Relative to K-Block TM
RF Blocking Strength Relative to K-Block TM
100
100
100.0
100.0
80
80
60
60
50.0
45.6
40
40
20
20
22.8
10.3
9.9
7.7
0
0
K Block
Native Mouse IgG
Competitor Blocker #1
Competitor Blocker #2
K Block
Native Mouse IgG
Competitor Blocker #1
Competitor Blocker #2
A double mouse monoclonal sandwich assay was used to compare the performance of Meridian Bioscience’s K-Block ™ (100% animal free, recombinant blocker, MLS Cat No. BN1200) against serum-derived purified native mouse IgG and two competitor’s recombinant mouse IgG HAMA blockers. (A) Human HAMA-positive or (B) RF-positive serum was preincubated with the individual blockers for 20 minutes at room temperature. Blocker concentrations ranged from 0.0001-1.25 µ g/mL for the HAMA assay and 0.01-80 µ g/mL for the RF assay. The effectiveness of each blocker was determined by comparing the relative suppression of the HAMA signal to the signal of samples with no blocker. The results demonstrate that at higher concentrations K-Block is as effective as the other blockers and at lower concentrations, K-Block was more effective than native mouse IgG and the two competitor’s recombinant HAMA blockers.
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