Meridian K Block Flyer

Performance Data K-Block TM demonstrates superior HAMA and RF blocking over Mouse IgG and other commercial HAMA blockers.

A.

B.

Relative HAMA Blocking Capacity

Relative RF Blocking Capacity

100

100

K-Block TM

K-Block TM

Native mouse IgG

Native mouse IgG

80

80

Competitor Blocker #1 Competitor Blocker #2

Competitor Blocker #1 Competitor Blocker #2

60

60

40

40

20

20

0

0

0.0001

0.001

0.01 Blocker Concentration ( µ g/ml)

0.1

1

0.01

0.1

Blocker Concentration ( µ g/ml) 1

10

100

HAMA Blocking Strength Relative to K-Block TM

RF Blocking Strength Relative to K-Block TM

100

100

100.0

100.0

80

80

60

60

50.0

45.6

40

40

20

20

22.8

10.3

9.9

7.7

0

0

K Block

Native Mouse IgG

Competitor Blocker #1

Competitor Blocker #2

K Block

Native Mouse IgG

Competitor Blocker #1

Competitor Blocker #2

A double mouse monoclonal sandwich assay was used to compare the performance of Meridian Bioscience’s K-Block ™ (100% animal free, recombinant blocker, MLS Cat No. BN1200) against serum-derived purified native mouse IgG and two competitor’s recombinant mouse IgG HAMA blockers. (A) Human HAMA-positive or (B) RF-positive serum was preincubated with the individual blockers for 20 minutes at room temperature. Blocker concentrations ranged from 0.0001-1.25 µ g/mL for the HAMA assay and 0.01-80 µ g/mL for the RF assay. The effectiveness of each blocker was determined by comparing the relative suppression of the HAMA signal to the signal of samples with no blocker. The results demonstrate that at higher concentrations K-Block is as effective as the other blockers and at lower concentrations, K-Block was more effective than native mouse IgG and the two competitor’s recombinant HAMA blockers.

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02/23

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