Antibody and Antigen Detection Assays Hepatitis C (HCV)
REAGENTS FOR SEROLOGY TESTING (Continued)
The HCV antibody test is commonly used to screen individuals with risk factors, people who have symptoms associated with hepatitis or liver disease, or those who have been exposed to the virus. It is generally followed by a confirmatory nucleic acid test to detect HCV RNA, which indicates an active viral infection. The confirmatory test is essential because between 15 and 50% of people with HCV antibodies will have spontaneously cleared HCV infection during the first six months after exposure and do not have chronic infection. Anti-HCV persists indefinitely in patients who develop chronic infection, although antibodies may become undetectable in hemodialysis patients or in cases of profound immunosuppression. HCV Antibody Test
HCV Core Recombinant Antigens
Suitable for ELISA and Western Blot Antibody Detection Assays
VTI710
• Represents a.a. 1-120 of HCV core (nucleocapsid protein p22), fused with a 6-His tag • Produced in P. pastoris • Molecular weight of ~19 kDa (monomer) • Buffer: 0.05 M Sodium Phosphate, 0.15 M NaCl, pH 7.0 • Represents a.a. 2-192 of HCV core, genotype 1, fused with a Beta- galactosidase taq at the N-terminus • Produced in E. coli (>95% pure) • Buffer: 8 M Urea, 20 mM Tris-HCl, pH 8.0, 10 mM beta-Mercaptoethanol
Suitable for ELISA, Western Blot, Colloidal Gold and Latex Bead Antibody Detection Assays
R8A115
HCV Capsid Recombinant Antigen
• Represents subtype 1a • Produced in E. coli (>70% pure) • Molecular weight of 33.4kDa • Buffer:4 M Urea • Represents subtype 1b • Produced in E. coli (>70% pure) • Molecular weight of 33.4kDa • Buffer:4 M Urea • Represents.subtype 2a • Produced in E. coli (>70% pure) • Molecular weight of 33.4kDa • Buffer: 4 M Urea • Represents.subtype 3a • Produced in E. coli (>70% pure) • Molecular weight of 33.4kDa • Buffer: 4 M Urea
BN1031
BN1033
Suitable for ELISA and Lateral Flow Antibody Detection Assays
BN1034
BN1035
15
Hepatitis- Reagents for Assay Development
Powered by FlippingBook