Molecular Reagents Optimized RT-qPCR Master Mix
MDX025 Low LOD 1-Step RT-qPCR Mix Suitable for detecting RNA and DNA viruses at very low levels for applications such as blood bank or transplant viral testing. Novel buffer system optimized for multiplex assays. Perform first-stand DNA synthesis and qPCR in a single tube.
Product Performance Data: Low Limit of Detection (LOD) for both RNA and DNA Viruses
Amplification Curves Amplification Curves HIV-2 (5 IU/mL)
Amplification Curves plification Curves HCV (30 IU/mL) Amplification Curves
23.688 23.079 23.079 23.6 8
40.432
21.688 21.079 21.079 21.6 8
36.932
19.688 19.079 19.079 19.6 8
33.432
17.688 17.079 17.079 17.6 8
29.932
15.688 15.079 15.079 15.6 8
26.432
13.688 13.079 13.079 13.6 8
22.932
11.688 11.079 11.079 1.6 8
19.432
9.688 9.079 9.079 9.6 8
15.932
7.688 7.079 7.079 7.6 8
12.432
5.688 5.079 5.079 3.688 3.079 3.079 3.6 8 1.688 1.079 1.079 1.6 8 5.6 8
8.932
5.432
1.932
2 4 6 8 10 2 4 6 8 10 12
12 12
14 14 20 14
16 16
18 18 26
20 20
22 22
24 24 24
26 26
28 28
30 30
32 32 32
34 34 34
36 36 36
38 38 38
40 40 40
42 42 42
44 44 4
10 4 16
18
22
24
28
22 30 3
34
36
38
40
42
44
2
4
6
8
12
16
18
20
26
28
30
2
4
6
8
10
2
4
6
8
10
12
14
16
18
20
22
24
26
28
30
32
34
36
38
40
42
44
Cycles
Cycles
Cycles
Cycles
Cycles
Amplification Curves HAV (5 IU/mL) Amplification Curves
Amplification Curves HBV (15 IU/mL) Amplification Curves
23.688
6.628
21.688
6.028
19.688
5.428
17.688
4.828
15.688
4.228
13.688
3.628
11.688
3.028
9.688
2.428
7.688
1.828
5.688
1.228
3.688
0.628
1.688
0.028
2
4
6
8
10
12
14
16
18
20
22
24
26
28
30
32
34
36
38
40
42
44
2
4
6
8
10
12
14
16
18
20
22
24
26
28
30
32
34
36
38
40
42
44
Cycles
Cycles
Cycles
Cycles
B19 (60 IU/mL)
Amplification Curves Amplification Curves HIV-1 (60 IU/mL)
Amplification Curves
Amplification Curves
63.075
43.077
57.075
39.077
35.077
51.075
31.077
45.075
27.077
39.075
23.077
33.075
19.077
27.075
15.077
21.075
11.077
15.075
7.077
9.075
3.077
3.075
2
4
6
8
10
12
14
16
18
20
22
24
26
28
30
32
34
36
38
40
42
44
2
4
6
8
10
12
14
16
18
20
22
24
26
28
30
32
34
36
38
40
42
44
Cycles
Cycles
Cycles
Cycles
Method: 24 replicates of purified blood viral extracts were tested in singleplex qPCR reactions using the Low LOD 1-Step RT-qPCR Mix at the indicated IU/mL. The singleplex reactions were HAV, HBV, HCV, HIV-1, HIV-2, Parvovirus B19. The amplification curve results illustrate that Low LOD 1-Step RT-qPCR Mix can be used with both RNA and DNA viruses as low as 5 IU/mL.
Sequencing Grade dNTPs Ready-to-use sequencing grade dNTPs available individually, as sets, or pre-blended dNTP mixes. Ultra-pure (>99% by HPLC) dNTPs supplied as lithium salts. Free of PCR inhibitors, DNase and RNase. For more information visit www.MeridianLifeScience.com.
Ordering information: USA E: info@meridianlifescience.com Toll free: +1 800 327 6299 UK E: info.uk@meridianlifescience.com Tel: +44 (0)20 8830 5300
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美国迈迪安生命科学公司 电子邮件: vivian.li@meridianlifescience.com 电话: +86-159-1103-0750
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