High inhibitor tolerance and fast amplification to deliver quicker time-to-results (TTR) in saliva, sputum and samples containing Universal Transport Media (UTM)
Lyo-Ready ™ Direct DNA LAMP Saliva (MDX134)
CMV
ADV
70
70
60
60
50
50
40
40
30
30
20
20
10
10
0
0
0% inhibitor
30% saliva
30% UTM + Sputum
30% VTM
NTC
0% inhibitor
30% saliva
30% UTM + Sputum
30% VTM
NTC
HSV2
Primers for CMV, ADV or HSV-2 were added to Lyo-Ready ™ Direct DNA LAMP Saliva (MDX134) and lyophilized ( blue ) or added to NEB WarmStart ® ( orange ) and kept as a liquid. The mixes were amplified using inactivated Cytomegalovirus (CMV), Adenovirus (ADV) or Herpes simplex virus 2 (HSV2). Reactions were prepared in the presence of 30% human saliva, 30% Universal Transport Medium (UTM) + an artificial sputum swab and Viral Transport Medium (VTM). All reactions were incubated at 65 °C for 60 minutes, and average time to results (TTR) was measured at 1:10 of end fluorescence. Error bars represent the standard deviation across four technical replicates. Results show that MDX134 ( blue ) has a shorter TTR than NEB WarmStart ® ( orange ) amplification across all three sample types. The data suggests that MDX134 performs better with faster amplification across the range of inhibitors.
70
60
50
40
30
20
10
0
0% inhibitor
30% saliva
30% UTM + Sputum
30% VTM
NTC
Lyophilized | NEB WarmStart ®
Lyo-Ready ™ Direct RNA/DNA LAMP Saliva (MDX135)
RSV
Primers for RSV were added to Lyo-Ready ™ Direct RNA/DNA LAMP Saliva (MDX135) and lyophilized ( blue ) or added to NEB WarmStart ® ( orange ) and kept as a liquid. The mixes were amplified using inactivated respiratory syncytial virus (RSV) RNA prepared with 30% human saliva, 30% Universal Transport Medium (UTM) with/without an artificial sputum swab and Viral Transport Medium (VTM). All reactions were incubated at 65 °C for 60 minutes, and the average time to results (TTR) was measured at 1:10 of end fluorescence. Error bars represent the standard deviation across six technical replicates. Results show that MDX135 ( blue ) has a shorter TTR than NEB WarmStart ® ( orange ) amplification across all sample types. The data suggests that MDX135 performs better with faster amplification across the range of inhibitors.
70
60
50
40
30
20
10
Lyophilized | NEB WarmStart ®
0
0% inhibitor
30% Saliva
30% UTM
30% VTM
30% Sputum in UTM
30% Sputum
NTC
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