Maintains consistent performance in samples containing up to 60% saliva
Lyo-Ready ™ Direct RNA/DNA LAMP Saliva (MDX135)
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Lyo-Ready ™ Direct RNA/DNA LAMP Saliva (MDX135) was lyophilized with Mycobacterium tuberculosis (MTB) primers and resuspended with increasing amounts of human saliva containing M. tuberculosis (from 10% - 72%). Reactions were incubated at 65 °C for 60 minutes, and TTR was measured at 1:10 of end fluorescence. The results illustrate that Lyo-Ready ™ Direct RNA/DNA LAMP Saliva maintains a robust performance up 60% saliva, and demonstrates consistently late NTCs.
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Lyophilized format maintains a stable shelf-life for more than 12 months
Lyo-Ready™ Direct RNA/DNA LAMP Saliva (MDX135)
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Freshly prepared Lyo-Ready ™ Direct RNA/DNA LAMP mix (MDX135) ( red ) was compared to lyophilized mix that had been incubated for 1 month at 37°C in an accelerated stability study ( blue ). Both mixes were amplified using PMSD4 3T3 mouse cell primers and reactions were incubated at 65 °C for 60 minutes. TTR was measured at 1:10 of end fluorescence and error bars represent the standard deviation across six technical replicates. Results suggest that the lyophilized mix retains full activity following accelerated stability tests with a projected stability of greater than 12 months at ambient temperature.
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