Hormones & Steroids

Parathyroid Hormone (PTH)

Parathyroid Hormone (PTH) is secreted by the parathyroid gland and plays an important role in bone remodeling. It also works together with Vitamin D to maintain healthy bones. PTH is secreted in response to low calcium levels as a 118 amino acid polypeptide that undergoes two successive cleavages to yield an 84 amino acid biologically active hormone. It has a very short half-life of less than five minutes and breaks down into various fragments of which the biological significance remains to be defined. A PTH blood test is useful in the differential diagnosis of overactive parathyroid glands (hyperparathyroidism). Primary hyperparathyroidism is most often caused by a benign tumor in one or more of the parathyroid glands and patients with this condition have high PTH and calcium levels. Secondary hyperparathyroidism is often seen in patients with chronic renal failure (CRF). The kidneys fail to excrete sufficient phosphate, and the parathyroid gland secretes PTH in an effort to lower calcium levels to balance the calcium-phosphate ratio. Tertiary hyperparathyroidism occurs when CRF causes a severe imbalance in the calcium- phosphate ratio, leading to very high PTH production that results in hypercalcemia. Current blood tests generally measure intact PTH by ELISA. Most assays use two antibodies in sequence, the first recognizing the N-terminal and the second the C- terminal.

Reagents for Immunoassay Development

E01306M

MAb to PTH (a.a. 1-34) • Reacts with a.a. 1-34 of human PTH

Suitable for use in ELISA & RIA

E24101M

MAb to PTH (a.a. 53-68) • Specific for human PTH peptide a.a. 53-68 • Does not cross-react with Synthetic human PTH peptide (a.a. 1-10, a.a. 1-34 and a.a. 1-38) • Lyophilized MAb to PTH (a.a. 53-84) • Does not cross react with synthetic human PTH peptide (a.a. 1-10, a.a. 1-34 and a.a. 1-38) • Produced in Cell Culture • Lyophilized from 0.1 M Phosphate Buffered Saline, pH 7.4

Suitable for use in ELISA, IHC & Immunoluminetric

E24150M

Suitable for use in ELISA & IHC

19

Endocrine Disorders - Reagents for Assay Development

Powered by