Thyroid Peroxidase (TPO)
Thyroid Peroxidase, also known as Thyroperoxidase (TPO), is an enzyme involved in thyroid hormone synthesis, catalyzing the oxidation of iodide on tyrosine residues in thyroglobulin for the synthesis of triiodothyronine (T3) and thyroxine (T4). It is one of the most important thyroid gland antigens and along with thyroglobulin (Tg), it is targeted by the immune system in several autoimmune thyroid diseases. Anti-TPO assays are useful in differentiating thyroid autoimmune disorders from non- autoimmune goiter or hypothyroidism. Anti-TPO antibodies are detectable in most autoimmune disorders including Hashimoto thyroiditis and Grave’s disease and may also be found in patients with non-thyroid autoimmune diseases such as pernicious anemia, type I diabetes, or other disorders that activate the immune system. Many clinical endocrinologists use the TPO antibody test as a diagnostic tool in deciding whether to treat a patient with subclinical hypothyroidism, as the presence of TPO antibodies is associated with an increased risk of developing overt hypothyroidism.
Reagents for Immunoassay Development
MAb to TPO • Recognizes native and recombinant TPO • Also suitable for WB • Capture Antibody MAb to TPO • Recognizes native and recombinant TPO • Also suitable for WB • Alternative Capture Antibody MAb to TPO • Recognizes native and recombinant TPO • Detection Antibody
TPO Recombinant Antigen • Produced in Sf9 cells • >96% pure (SDS-PAGE)
Suitable for use in ELISA & WB
• Buffer: 40 mM Tris-HCl, pH 7.6 containing 144 mM Sodium Chloride, 0.08 mM Potassium Iodide and 20%Glycerol TPO Recombinant Antigen • Represents the soluble extracellular domain of human TPO (a.a. 19-846) • Produced in insect cells • >95% pure (SDS-PAGE) • Lyophilized (from 20 mM Tris-HCl, pH 8.0 containing 250 mM Sodium Chloride, 0.1 mM Potassium Iodide and 5% D-Mannitol)
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