Hormones & Steroids

Thyroid-stimulating hormone (TSH) Thyroid-stimulating hormone (TSH) is a pituitary hormone that regulates thyroid function in conjunction with thyrotropin releasing hormone (TRH). Specifically, TSH induces production of Thyroxine (T4) which is converted triiodothyronine (T3) and is responsible for stimulating the metabolism. TSH is secreted throughout life but reaches high levels during the periods of rapid growth and development, as well as in response to stress. The release of TSH from the pituitary is regulated by the concentration of T3 and T4 in the blood. When T3 and T4 concentrations are low, the production of TSH is increased, and, conversely, when T3 and T4 concentrations are high, TSH production is decreased. Any imbalance in this negative feedback loop can be the indication of a thyroid disease, such as hyperthyroidism or hypothyroidism. The most common cause of hypo- and hyperthyroidism is autoimmune disease. In Hashimoto’s disease, the immune system attacks the thyroid and causes hypothyroidism. Conversely, Grave’s disease causes an overactive thyroid leading to hyperthyroidism. TSH serum measurements are used as a first line screening assay for hypo- and hyperthyroidism. The capability of a TSH assay to distinguish between normal and subnormal concentrations is critical for this thyroid testing strategy. Quantification of TSH at a lower value of 0.01 mlU/L yields information that is useful to clinicians referring patients with subnormal TSH concentrations. Reagents for Immunoassay Development

MAT04-003

MAb to TSH • Specific to intact TSH. Reacts with TSH through the alpha subunit/hinge region • Does not react with free beta subunit • Has limited reactivity to free alpha subunit MAb to TSH • Cross-reactivity: FSH (0.7%), LH (0.5%), TSH alpha (1.5%) PAb to Human TSH beta • <0.1% cross-reactivity with alpha hCG, LH, FSH, growth hormone, and prolactin • Produced in Goat TSH Native Antigen • Produced from human pituitary glands • >95% pure (SDS-PAGE) • Contaminants: FSH (< 0.02%), LH (<0.05%), hGH (<0.05%), Prolactin (<0.05%) • Lyophilized from 50 mM Ammonium Bicarbonate

MAT04-410

D92409G

Suitable for use in ELISA

A75510H

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Endocrine Disorders - Reagents for Assay Development

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