Blockers Practical Guide

Solid Phase Blockers Solid phase blocking agents are designed to saturate unoccupied binding sites on the solid phase to prevent non-specific binding. Typically blocking buffers are able to block both hydrophobic and hydrophilic sites on the solid phase. In addition, they can serve as stabilizing agents and prevent denaturation as proteins react at the surface of the solid phase.

Specificity

Cat Number

Host / Source

Format

Blocking Buffer for ELISA

J82100B J82300B J16430D J16200D A64801B A51300B

n/a n/a n/a n/a

n/a

Blocking Buffer for Lateral Flow Assays, PBS based

Liquid Liquid Liquid

Coating Stabilizer & Blocking Buffer Conjugate Stabilizer Diluent, HRP

BSA, Standard Powder Gamma Globulin, Bovine Bovine Serum Albumin (BSA)

Bovine

Purified Purified Aff.Pur.

Bovine Serum

C5BP01-188

Chicken

IgG Absorbents IgG absorbents are designed for the removal of human IgG and Ig / rheumatoid factor (RF) complexes from serum and plasma prior to testing for specific IgM antibodies in ELISA or other immunoassays. Removal of IgG interference has been demonstrated to significantly increase the sensitivity of IgM detection.

Specificity

Cat Number

Host / Source

Format Purified

IgM Assay Diluent

8120

Goat Goat Goat Goat

IgG (Fc) IgG (Fc) IgG (Fc)

L15406G

Monospecific

G5G16-048

Aff. Pur. Purified

L62540G

Active HAMA & RF Blocker In double mouse monoclonal sandwich assays, an active blocker against human anti-mouse antibodies (HAMA) and rheumatoid factor (RF) should be used to prevent non-specific interference leading to false assay results. TRU Block is a proprietary active blocker that can significantly reduce assay interference and improve assay results. It can be used at a lower concentration than passive blocking reagents such as mouse IgG.

Specificity

Cat Number

Host / Source

Format Purified Purified Purified Purified

K-Block™ 100% Animal Protein-Free, Active Blocker

BN1200

Confidential Confidential

TRU Block™ Ultra, Active Blocker TRU Block™, Active Blocker TRU Block™ 3, Active Blocker

8000

A66800H A66803H

Mouse & Confidential Mouse & Confidential

HAMA & RF Reagents HAMA (human anti-mouse antibodies) or rheumatoid factor positive patient samples that can be used to test the effectiveness of a blocking agent in any type of assay.

Specificity

Cat Number

Host / Source Human Plasma Human Plasma

Format

Rheumatoid Factor (RF) Control Serum Rheumatoid Factor (RF) Control Serum

A01270H A12916H

Neat Neat