LAMP & RT-LAMP Mixes
Over the past decade, isothermal amplification techniques such as HCA, MDA and loop-mediated isothermal amplification (LAMP) have played an increasingly important role in point-of-care (POC) diagnostics. LAMP offer several advantages over qPCR and other amplification techniques in terms of sample-to-answer time, sensitivity, specificity, cost and robust amplification at a constant temperature. It is also simpler to operate, using less expensive, portable equipment, making it ideal for field-deployable diagnostics in resource-limited settings. LAMP based assays have been used for numerous applications including the detection of pathogens such as salmonella and malaria, genetically modified crop contamination and in forensics to specifically detect human DNA. In addition, RT-LAMP is widely used for screening for a number of pathogens, particularly RNA viruses, such as influenza, rotavirus, hepatitis C, West Nile fever, Dengue virus, Ebola virus and most recently, SARS-CoV-2. Meridian has developed a blend of enzymes, dNTPs, Mg 2+ , excipients and stabilizers that can be used with customer-selected primers and probes for creating fast, robust DNA or RNA LAMP assays. However, a major factor limiting the accessibility of POC testing is cold chain management which is required to preserve the integrity of the assay reagents. Meridian’s Lyo-Ready ™ and Air-Dryable ™ LAMP Mixes have been designed to overcome this challenge.
Key Features
• Ideal for POC diagnostic or automated high-throughput instruments • Available in universal or Specimen-specific ™ formulations for blood, saliva, urine or stool and suitable for direct detection assays from crude samples
• Fully optimized, concentrated 4x master mixes, just add primers and probe • Contains all the required excipients for subsequent lyophilization or air-drying • Shelf-life greater than 12 months after lyophilization or air-drying
Blood-specific Mixes
Blood
Meridian’s Lyo-Ready ™ Direct DNA and DNA/RNA LAMP Blood Mixes are the newest solution for assays requiring high sensitivity and fast time to results and can detect DNA or RNA down to single copies in blood, serum or plasma samples. The mixes are designed to be extremely inhibitor-tolerant, enabling direct detection using crudely processed samples, making them ideal for developing next-generation, culture-free assays detecting bacterial infections such as sepsis. They outperform other commercial LAMP mixes in terms of sensitivity, specificity and speed which is demonstrated by their quicker time-to- result (TTR) and lower non-specific amplification (NTC) across a range of targets.
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL 800 Rxns 50 mL 8,000 Rxns 5 mL 800 Rxns 50 mL 8,000 Rxns
Lyo-Ready Direct DNA LAMP Blood Mix
MDX124
Lyo-Ready Direct RNA/ DNA LAMP Blood Mix
MDX125
By using innovative ready-to-use isothermal master mixes such as Lyo-Ready ™ Direct DNA LAMP or RNA/DNA LAMP Blood mix, assay development time for a new ultra-sensitive direct blood assay is rapid, simple and efficient. Perfect for increasing your competitive edge and capturing value faster in the rapidly growing point-of-care market.
www.meridianbioscience.com/lifescience
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