LAMP & RT-LAMP Mixes
Superior Specificity and Quicker Time to Detection for Viral and Bacterial Targets
A) Lyo-Ready™ DNA LAMP Blood (MDX124)
B) Lyo-Ready™ RNA/DNA LAMP Blood (MDX125)
60
60
50
50
40
40
30
30
20
20
10
10
0
0
Sample
NTC
Sample
NTC
Sample
NTC
Sample
NTC
Sample
NTC
Sample
NTC
Sample
NTC
Sample
NTC
Sample
NTC
Sample
NTC
HBV
HSV2
EBV
T. vaginalis
M. pneumoniae
HCV
HIV-1
DENGUE 1
WNV
Zika
Lyo-Ready™ Direct DNA LAMP Blood | NEB WarmStart® | Thermo SuperScript™
(A) Lyo-Ready™ Direct DNA LAMP Blood (MDX124, red ) was lyophilized with primers for Trichomonas vaginalis , Hepatitis B Virus (HBV), Herpes Simplex Virus 2 (HSV2), Epstein-Barr virus (EBV), Mycoplasma pneumoniae and (B) Lyo-Ready™ Direct RNA/DNA LAMP Blood (MDX125, red ) was lyophilized with primers for Hepatitis C Virus (HCV), HIV-1, Dengue type 1, West Nile Virus (WNV) or Zika. The performance of the mixes were compared against NEB WarmStart® LAMP kit ( orange ) and Thermo SuperScript™ IV RT-LAMP Master Mix ( black ) using the same primers and DNA/ RNA. Reactions were incubated at 65°C for 60 minutes. Error bars represent the standard deviation across three technical replicates. Both Lyo- Ready™ Direct LAMP Blood mixes delivered faster target amplification (TTR) with higher specificity, as demonstrated by the longest delay in no-template control (NTC) amplification across range of pathogenic DNA and viral RNA targets.
High InhibitorTolerance in the Presence of Whole Blood K2-EDTA or Plasma
(A) Lyo-Ready™ DNA LAMP Blood (MDX124)
(B) Lyo-Ready™ RNA/DNA LAMP Blood (MDX125)
60
60
50
50
40
40
30
30
20
20
10
10
0
0
HBV + 5% Blood
EBV + 20% Blood
P. malaria + 5% Blood
HSV2 + 20% Plasma
M. pneumoniae + 5% Blood
Dengue 1 + 10% Blood
HCV + 10% Blood
WNV + 5% Blood
ZIKV + 5% Blood
HIV-1 Plasma extract
Lyo-Ready™ Direct DNA LAMP Blood | NEB WarmStart® | Thermo SuperScript™
Lyo-Ready™ Direct DNA LAMP Blood (MDX124, red ) was lyophilized with primers for HSV2, EBV, HBV, P. malaria and M. pneumonia and Lyo- Ready™ Direct RNA/DNA LAMP Blood (MDX125, red ) was lyophilized with primers for Dengue Type-1, HCV, WNV, Zika or HIV-1 and has it performance tested against WarmStart® LAMP kit ( orange ) and SuperScript™ IV RT-LAMP Master Mix ( black ). Reactions were prepared in the presence of 5-20% Blood K2-EDTA or plasma extract. The data illustrates better performs with faster amplification across the range of inhibitors for both Lyo-Ready™ Direct LAMP Blood mixes, with earlier TTRs across a broad range of pathogenic targets.
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