Saliva-Specific Mixes
Saliva
Meridian’s new Lyo-Ready ™ Direct DNA LAMP and RNA/DNA LAMP Saliva mixes are the newest advancement in molecular reagent technology and combine the benefits of a highly inhibitor-tolerant mix, with robust isothermal amplification, to enable direct detection using crudely processed samples. The mixes are uniquely designed to overcome saliva and sputum inhibitors and they do not require a DNA or RNA purification step to and purify a patient sample. In addition, the 4x
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CAT NO. VOLUME REACTIONS
5 mL 800 Rxns 50 mL 8,000 Rxns 5 mL 800 Rxns 50 mL 8,000 Rxns
Lyo-Ready Direct DNA LAMP Saliva Mix
MDX134
Lyo-Ready Direct RNA/ DNA LAMP Saliva Mix
MDX135
concentrated master mixes are formulated with lyo-excipients which are optimized for freeze-drying to create ambient- temperature stable assays. By using innovative ready-to-use isothermal master mixes such as Lyo-Ready ™ Direct DNA LAMP or RNA/DNA LAMP Saliva mix, assay development time for a new ultra-sensitive direct saliva assay is rapid, simple and efficient.
Superior assay specificity using samples containing 15% sputum
Lyo-Ready™ Direct DNA LAMP Saliva (MDX134)
Amplification of Mycoplasma pneumoniae genomic DNA (200 copies) was compared using Lyo-Ready™ Direct DNA LAMP Saliva (MDX134) against NEB WarmStart® in samples containing 0% or 15% artificial sputum. Reactions were incubated at 65 °C for 60 minutes, and TTR was measured at 1:10 of end fluorescence. No NTCs were detected in this assay. Lyo-Ready™ Direct DNA LAMP Saliva (MDX134) with 0% ( light blue ) and 15% ( dark blue ) artificial sputum demonstrated stronger inhibitor tolerance, retaining its fast TTR in the presence of 15% artificial sputum. In contrast, NEB WarmStart® with 0% artificial sputum ( orange ) amplified slower, as shown by a later TTR, than either reaction using Lyo-Ready™ Direct DNA LAMP Saliva. In addition, in samples containing 15% artificial sputum ( red ), NEB WarmStart® had a delayed amplification of 40 min and the longest TTR of all the reactions.
MDX134 0% sputum | MDX134 15% sputum | NEB WarmStart® 0% sputum | NEB WarmStart® 15% sputum
Maintains consistent performance in samples containing up to 60% saliva
Lyo-Ready ™ Direct RNA/DNA LAMP Saliva (MDX135)
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Lyo-Ready™ Direct RNA/DNA LAMP Saliva (MDX135) was lyophilized with Mycobacterium tuberculosis (MTB) primers and resuspended with increasing amounts of human saliva containing M. tuberculosis (from 10%- 72%). Reactions were incubated at 65 °C for 60 minutes, and TTR was measured at 1:10 of end fluorescence. The results illustrate that Lyo-Ready™ Direct RNA/ DNA LAMP Saliva maintains a robust performance up 60% saliva and demonstrates consistently late NTCs.
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