LAMP & RT-LAMP Mixes
Urine-Specific Mixes
Urine
Urine has long been used as a diagnostic sample type for sexually transmitted infections (STIs) such as chlamydia, gonorrhoea, HSV and HPV, among many others. It has also been used to monitor hormone and metabolic disorders, renal diseases, bladder cancer, urinary tract infections (UTIs) and for monitoring recreational drug use. Urine is an ideal clinical specimen because it is excreted in large quantities, it is non-invasive, and it can be self-sampled. Lyo-Ready Direct DNA LAMP and Direct RNA/DNA LAMP Urine mixes are designed to deliver fast and sensitive amplification of DNA or RNA using direct detection methods. Samples do not require nucleic acid extraction and can be used directly on the assay. The mixes also contain excipients and an optimized buffer system that is compatible with lyophilization to create ambient-temperature stable assays which have more than two-year shelf-life.
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL 800 Rxns 50 mL 8,000 Rxns 5 mL 800 Rxns 50 mL 8,000 Rxns
Lyo-Ready Direct DNA LAMP Urine Mix
MDX154
Lyo-Ready Direct RNA/DNA LAMP Urine Mix
MDX155
Results Delivered in Half the Time with Samples Containing 20-30% Urine Lyo-Ready DNA LAMP Urine (MDX154) HSV1 + 30% Urine
60
50
40
30
N. gonorrhoea + 30% Urine
20
10
0
N.gonorrhoeae +20% Urine
L. interoggans +30% Urine
M. genitalium +20% Urine
C. trachomatis +30% Urine
T. vaginalis +30% Urine
HSV1 +20% Urine
Lyophilized | NEB WarmStart® | SuperScript™
Lyo-Ready Direct DNA LAMP Urine (MDX154, red ) was lyophilized with primers for Neisseria gonorrhoeae, Leptospira interrogans, Mycoplasma genitalium, Chlamydia trachomatis, Trichomonas vaginalis and Herpes Simplex Virus 1 (HSV1) The performance of the mixes was compared against liquid formats of NEB WarmStart® LAMP kit ( orange ) and Thermo SuperScript™ IV RT-LAMP Master Mix ( black ) using the same primers and DNA/ RNA. Reactions were prepared in the presence of 5-30% human urine. All reactions were incubated at 65 °C for 60 minutes. Error bars represent the standard deviation across four technical replicates. Results show that MDX154 has earlier TTRs across a broad range of pathogenic DNA and viral RNA targets. The data illustrates Lyo-Ready Direct DNA LAMP Urine (MDX154) performs better and with faster amplification across the range of inhibitors compared to the other mixes.
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