Meridian Specimen-specific qPCR & LAMP Master Mixes

Results Delivered in Half the Time with Samples Containing 20-30% Urine

Dengue Type-1 + 20% Urine

Lyo-Ready™ RNA/DNA LAMP Urine (MDX155)

Chikungunya + 5% Urine

CHIKV + 5% Urine

HCV + 5% Urine

ZIKV + 20% Urine

WNV + 5% Urine

DENV + 20% Urine

Lyophilized | NEB WarmStart® | SuperScript™

Lyo-Ready™ Direct RNA/DNA LAMP Urine (MDX155, red ) was lyophilized with primers for either Chikungunya Virus (CHIKV), Hepatitis C Virus (HCV), Zika, West Nile Virus (WNV) or Dengue Type-1. The performance of the mixes was compared against liquid formats of NEB WarmStart® LAMP kit ( orange ) and Thermo SuperScript™ IV RT-LAMP Master Mix ( black ) using the same primers and DNA/RNA. Reactions were prepared in the presence of 5-30% human urine. All reactions were incubated at 65 °C for 60 minutes. Error bars represent the standard deviation across four technical replicates. Results show that MDX155 has earlier TTRs across a broad range of pathogenic DNA and viral RNA targets. The data illustrates Lyo-Ready™ Direct RNA/DNA LAMP Urine (MDX155) performs better and with faster amplification across the range of inhibitors compared to the other mixes.

www.meridianbioscience.com/lifescience

Powered by