LAMP & RT-LAMP Mixes
Universal Mixes
Universal
Meridian’s Lyo-Ready ™ and Air-Dryable ™ LAMP and RT- LAMP master mixes are the market-leading solution for molecular diagnostic assays requiring fast cycling, superior sensitivity, high inhibitor tolerance and ambient temperature stability. They are 4x concentrated, glycerol-free and contain specialized excipients that are compatible with lyophilization or air-drying to create ambient-temperature stable assays, or they can be used as a wet mix. They are highly inhibitor- tolerant and ultra-sensitive making them ideal for point-of- care or machine-automated multiplexing assays. As universal mixes, they are highly resistant to various LAMP inhibitors and can be used across a range of patient sample types.
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL 800 Rxns 50 mL 8,000 Rxns 5 mL 800 Rxns 50 mL 8,000 Rxns 5 mL 800 Rxns 50 mL 8,000 Rxns 5 mL 800 Rxns 50 mL 8,000 Rxns
Air-Dryable LAMP Mix
MDX119
Air-Dryable RT-LAMP Mixes
MDX118
Lyo-Ready LAMP Mix
MDX097
Lyo-Ready RT-LAMP 1-Step Mix
MDX108
Fast Polymerization for Quick Sample-to-AnswerTime
Lyo-Ready™ LAMP Mix (MDX097)
The average time-to-result (TTR) for Lyo-Ready ™ LAMP Mix (MDX097, orange ) and NEB WarmStart® ( blue ) were compared for three genes, 9TV10 from Trichomonas Vaginalis (100 copies), hBRCA1 from human genomic DNA (200 copies) and MTB1 from Mycobacterium Tuberculosis (1,000 copies). The results demonstrate the faster speed of Lyo-Ready™ LAMP Mix, with earlier TTR values when compared to NEB WarmStart®. Reactions were incubated at 65°C for 60 min and TTR were measured at 1:10 of end fluorescence.
25.00
20.00
15.00
10.00
5.00
0.00
9TV10 Trichomonas vaginalis
hBRCA-1
MTB1
Human DNA
Mycobacterium tuberculosis
MDX097 | NEB WarmStart®
Mix performance is not impacted by lyophilization or subsequent room temperature storage
Lyo-Ready™ RT-LAMP 1 Step Mix (MDX108)
LAMP reactions in the presence ( green ) or absence ( teal ) of 10% UTM and sputum were performed in 5 replicates using PMSD4.1 primers and RNA to compare the performance of fresh (wet) Lyo-Ready™ RT-LAMP 1-Step Mix (MDX108) against lyophilized Lyo-Ready™ RT-LAMP 1-Step Mix, stored at 37ºC for 3 weeks. Time-to-result is set as the time at which the fluorescence crossed a threshold of 10% of maximal fluorescence. The data illustrate that the lyophilization process does not affect the stability of the Lyo-Ready™ RT-LAMP Mix and at a storage temperature of 37ºC it can be extrapolated that the lyophilized mix is stable at room temperature for up to 12 months.
30
20
10
0
Wet Mix
Lyophilized
Wet Mix
Lyophilized
No Inhibitor
10% UTM + Sputum
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