Superior sensitivity with shorter time-to-results (TTR) A) Air-Dryable ™ DNA LAMP (MDX119)
B) Air-Dryable ™ RNA/DNA LAMP (MDX118)
50
80
40
60
30
40
20
20
10
0
0
1000 copies/rxn 100 copies/rxn
20 copies/rxn
NTC
1000 copies/rxn 500 copies/rxn 100 copies/rxn
10 copies/rxn
(A) Conserved regions from the S and P genes of Hepatitis B Virus (1,000, 100 and 20 copies respectively) were amplified using Air-Dryable™ DNA LAMP (MDX119) in an air-dried ( red ) or liquid (blue) format or using NEB WarmStart® ( orange ). (B) Emesvirus (MS2) was amplified using Air-Dryable™ RNA/DNA LAMP (MDX118) in an air-dried ( red ) or liquid ( blue ) format or using NEB WarmStart® ( orange ). Reactions were incubated at 65°C for 60 min and TTR were measured at 1:10 of end fluorescence. Error bars represent standard deviation over 4 technical replicates. At lower copy numbers, NEB WarmStart® ( orange ) exhibited significantly lower reaction reproducibility compared to Air-Dryable™ DNA LAMP Mix, and a lower TTR was observed for reactions using both Air-Dryable™ DNA LAMP Mix and Air-Dryable™ DNA/RNA LAMP Mix across all dilutions of the Hepatitis B gene and MS2-RNA respectively. The results therefore demonstrate greater sensitivity of Air-Dryable™ LAMP Mixes with shorter TTR across all template concentrations.
High reaction specificity with little-to-no non-specific amplification
Air-Dryable™ RNA/DNA LAMP (MDX118)
Air-Dryable™ RNA/DNA LAMP (MDX118, blue ) was compared to NEB WarmStart® ( orange ) for detection of EBV DNA (1,000 ipc) and MS2 RNA (1,000 ipc). Reactions were incubated at 65°C for 60 min and TTR were measured at 1:10 of end fluorescence. Amplification plots of 3 technical replicates demonstrate Air-Dryable™ RNA/DNA LAMP mix has earlier TTR and no non-specific amplification compared to NEB WarmStart® which has detectable amplification for non-template controls (NTC).
EBV-DNA
MS2-RNA
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