qPCR and RT-qPCR Mixes
Currently, qPCR/RT-qPCR is regarded as the ‘gold standard’ for the quantitative analysis of nucleic acids with diverse applications such as gene expression analysis for molecular diagnostics, detection of genetically modified organisms in food, and cancer phenotyping. Meridian’s Lyo-Ready™ and Air-Dryable™ qPCR and RT-qPCR master mixes are the market-leading solution delivering fast cycling times, superior sensitivity, and high inhibitor tolerance for all assay types.
Specimen-specific™ Mixes
Blood-Specific Mixes
Blood
Blood is one of the most common specimens used for laboratory diagnostic testing and it is useful for evaluating the function of vital organs and for diagnosing diseases such as bacterial and viral infections, cancer (liquid biopsy), cardiovascular disease, and metabolic disorders including diabetes. However, there are several PCR inhibitors such as immunoglobulin G, hemoglobin, lactoferrin and leukocyte DNA that are present in blood which impact a reaction’s efficiency. In addition, anticoagulants used to stabilize blood samples (e.g., EDTA, citrate or heparin) contain a number of inhibitors which cause interference in a PCR reaction.
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns
Air-Dryable Direct DNA qPCR Blood Mix, 4x
MDX092
Air-Dryable Direct RNA/ DNA qPCR Blood Mix, 4x
MDX121
Lyo-Ready Direct DNA qPCR Blood Mixes
MDX122
Lyo-Ready Direct RNA/DNA qPCR Blood Mixes
MDX123
Meridian’s Air-Dryable™ and Lyo-Ready™ direct blood mixes are unique by enabling direct amplification of target DNA or RNA from crudely processed blood, serum or plasma samples. They have been designed to efficiently amplify in the presence of inhibitors found in blood – no further optimization is required.
High reaction efficiency on plasma, serum and whole blood samples containing anticoagulants
Air-Dryable™ Direct DNA qPCR Blood (MDX092)
Plasma
Serum
Air-dried | KAPA Probe Force | TaqPath™ ProAmp™
Plasmid DNA containing Epstein-Barr virus was spiked into 10% serum or 10% K2-EDTA plasma and amplified in a triplex reaction using air-dried MDX092 format ( red ) and kits from KAPA Probe Force ( green ) andTaqPath™ ProAmp™ ( black ).The results illustrate higher end florescence and better sensitivity with MDX092 than with mixes from KAPA andThermo.
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