High tolerance to whole blood stabilized with anticoagulants (K2-EDTA, sodium heparin, and sodium citrate)
A) Air-Dryable™ Direct DNA qPCR Blood (MDX092)
B) Air-Dryable™ Direct RNA/DNA qPCR Blood (MDX121)
KAPA Probe Force
TaqPath™
QuantaBio
TaqPath™ 1-Step
K2-EDTA | Sodium heparin | Sodium citrate
Anticoagulants and high concentrations of whole blood are known to inhibit qPCR efficiencies. 0% 2%, 5% and 20% human whole blood in the presence of K2-EDTA ( orange ), sodium heparin ( green ) and sodium citrate ( grey ) was tested with (A) Air-Dryable™ Direct DNA qPCR Blood (MDX092) against mixes from KAPA Probe Force (Roche) and TaqPath™ (Thermo) and (B) Air-Dryable™ Direct RNA/DNA qPCR Blood (MDX121) against mixes from UltraPlex™ (QuantaBio) and TaqPath™ 1-Step (Thermo). The results demonstrate that the reaction efficiencies of the Air-Dryable™ Direct Blood mixes are higher both in the presence of anticoagulants and high concentrations of blood than of other suppliers’ mixes. High multiplexing capacity enables the detection of multiple analytes from crude, inhibitor-rich plasma, serum and whole blood samples
Lyo-Ready™ Direct DNA qPCR Blood (MDX122)
S. aureus
P. falciparum
Epstein-Bar virus
Lyo-Ready™ Direct DNA qPCR Blood | KAPA Probe Force | TaqTath™ ProAmp™ Mix
Three diagnostic DNA targets ( Staphylococcus aureus , Plasmodium falciparum and Epstein-Bar virus) were amplified in a triplex reaction using lyophilized Lyo-Ready™ Direct DNA qPCR Blood (MDX122, red ), KAPA Probe Force ( green ) or TaqPath™ ProAmp™ Mix ( black ) in the presence of 10% EDTA plasma or 10% serum. The results illustrate that Lyo-Ready™ Direct DNA qPCR Blood has higher multiplexing capacity and performance than KAPA Probe Force and TaqPath™ ProAmp™ Mix.
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