qPCR and RT-qPCR Mixes
Saliva-Specific Mixes
Saliva
Saliva has been extensively studied as a diagnostic specimen due to its non-invasive and simple sample collection/logistics. Similar to serum, saliva contains hormones, antibodies, growth factors, enzymes, and microbes that can be used as biomarkers for disease detection. However, there have been challenges adopting saliva for molecular diagnostics due to the low concentration of analytes and high concentration of PCR inhibitors. Meridian’s new Air-Dryable™ and Lyo-Ready direct saliva mixes are unique in that they have been designed to specifically overcome the inhibitors found in saliva or sputum samples. As a result, they are capable of highly sensitive amplification using crudely processed samples or extracted DNA or RNA in multiplex detection assays.
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns
Air-Dryable Direct DNA qPCR Saliva Mixes
MDX130
Air-Dryable Direct RNA/ DNA qPCR Saliva Mixes
MDX131
Lyo-Ready Direct DNA qPCR Saliva Mixes
MDX132
Lyo-Ready Direct RNA/DNA qPCR Saliva Mixes
MDX133
Lyo-Ready dUTP Direct DNA qPCR Saliva, 4x
MDX332
Lyo-Ready dUTP Direct RNA/DNA qPCR Saliva, 4x
MDX333
Sensitive Detection in Multiplex Assays Using Saliva Samples (Up to 60% Human Saliva) or UniversalTransport Media (Up to 35% UTM)
Air-Dryable™ Direct DNA qPCR Saliva (MDX130)
Mycoplasma pneumoniae
ADV
CMV
0% human saliva | 10% human saliva | 20% human saliva | 60% human saliva
Mycoplasma pneumoniae genomic DNA, adenovirus (ADV) and cytomegalovirus (CMV) were amplified in a triplex qPCR assay in the presence of 0% ( black ), 10% ( red ), 20% ( blue ) and 60% ( grey ) homogenized human saliva. The results illustrate that the performance of the air-dried, Air-Dryable™ Direct DNA qPCR Saliva (MDX130) is not affected by increasing concentrations of human saliva.
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