qPCR and RT-qPCR Mixes
Urine-Specific Mixes
Urine
Urine is an ideal clinical specimen because it is excreted in large quantities, non-invasive, and can be self-sampled. Currently, urine specimens are used in the diagnosis and management of infectious diseases (including STDs), hormone and metabolic disorders, renal diseases, bladder cancer, urinary tract infections (UTIs) and for monitoring recreational drug use. However, urine contains substances such as urea and nucleases that can damage DNA or inhibit a PCR reaction. Air-Dryable ™ and Lyo-Ready urine mixes are inhibitor-tolerant and designed for very fast, highly sensitive amplification of DNA and RNA directly from high concentrations of urine and are sensitive enough to detect arboviruses, such as Chikungunya virus. Patient urine samples can be used directly on the dried assay, and do not require nucleic acid purification.
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns
Air-Dryable Direct DNA qPCR Urine Mixes
MDX150
Air-Dryable Direct RNA/ DNA qPCR Urine Mixes
MDX151
Lyo-Ready Direct DNA qPCR Urine
MDX152
Lyo-Ready Direct RNA/DNA qPCR Urine
MDX153
Lyo-Ready dUTP Direct DNA qPCR Urine, 4x
MDX352
Enables Highly Sensitive Detection of Cancer Markers in Urine Samples
Air-Dryable™ Direct RNA/DNA qPCR Urine (MDX151)
CDC2
IGFBP5
MDK
MDK
Air-dried | Ultraplex (QuantaBio) ™
Three tumor-related mRNA markers (CDC2 kinase, IGFBP5 and MDK) were amplified from total RNA (from a bladder cancer patient) in a triplex reaction using air-dried Air-Dryable™ Direct RNA/DNA qPCR Urine ( red ) or UltraPlex™ (QuantaBio, grey ) in 10% human urine. The results illustrate that Air-Dryable™ Direct RNA/DNA qPCR Urine (MDX151) is able to detect cancer related RNA markers from urine faster and with high sensitivity compared to the other mix.
Superior Reaction Efficiency with Crude Urine Samples in a Multiplex Reaction
Air-Dryable™ Direct RNA/DNA qPCR Urine (MDX151)
Dengue
Zika
Chikungunya
MDX151 (Air-dried) | Reliance 1-Step Multiplex RT-qPCR Supermix | UltraPlex™ 1-Step ToughMix
Three viral RNA targets (Dengue, Zika and Chikungunya) were amplified in a triplex reaction using air-dried Air-Dryable™ Direct RNA/DNA qPCR Urine (MDX151, red ) and kits from Reliance 1-Step Multiplex RT-qPCR Supermix ( green ) and UltraPlex™ 1-Step ToughMix ( grey ) in the presence of 10% human urine. The results illustrate that dry Air-Dryable Direct RNA/DNA qPCR Urine has higher multiplexing capacity and reproducibility in the presence of 10% urine.
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