Specimen-specific ™ LAMP & RT-LAMP Mixes
Saliva-specific Mixes
Saliva
Saliva is ideal for use as a diagnostic specimen as it contains hormones, antibodies, growth factors, enzymes and microbes that can be used as biomarkers for disease detection. Saliva is easy-to-collect and allows non-invasive and simple sample collection/logistics, making it perfect for point of care testing. However, due to the low concentration of analytes and high concentration of inhibitors in saliva, its use in molecular diagnostics has been limited. Isothermal amplification such as LAMP is very tolerant to inhibitors and the simple workflow allows fast, sensitive, reliable results in clinical diagnosis.
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL 800 Rxns 50 mL 8,000 Rxns 5 mL 800 Rxns 50 mL 8,000 Rxns
Lyo-Ready Direct DNA LAMP Saliva Mix
MDX134
Lyo-Ready Direct RNA/DNA LAMP Saliva Mix
MDX135
Meridian’s new Lyo-Ready TM Direct DNA LAMP and RNA/DNA LAMP Saliva mixes are the newest advancement in molecular reagent technology and combine the benefits of a highly inhibitor-tolerant mix, with robust isothermal amplification, to enable direct detection using crudely processed samples. The mixes are uniquely designed to overcome saliva and sputum inhibitors and they do not require a DNA or RNA purification step to purify a patient sample. In addition, the 4x concentrated master mixes are formulated with lyo-excipients which are optimized for freeze-drying to create ambient-temperature stable assays. By using innovative ready-to-use isothermal master mixes such as Lyo-Ready TM Direct DNA LAMP or RNA/DNA LAMP Saliva mix, assay development time for a new ultra-sensitive direct saliva assay is rapid, simple and efficient. Perfect for increasing your competitive edge and capturing value faster in the rapidly growing point-of-care market.
High inhibitor tolerance and fast amplification to deliver quicker time-to-results (TTR) in saliva, sputum and samples containing Universal Transport Media (UTM)
Lyo-Ready™ Direct DNA LAMP Saliva (MDX134)
HSV2
CMV
ADV
70
70
70
60
60
60
50
50
50
40
40
40
30
30
30
20
20
20
10
10
10
0
0
0
0% inhibitor
30% saliva
30% UTM + Sputum
0% inhibitor
30% saliva
0% inhibitor
30% saliva 30% UTM + Sputum
30% VTM NTC
30% VTM
NTC
30% UTM + Sputum
30% VTM
NTC
Lyophilized | NEB WarmStart®
Primers for CMV, ADV or HSV-2 were added to Lyo-Ready™ Direct DNA LAMP Saliva (MDX134) and lyophilized ( blue ) or added to NEB WarmStart® ( orange ) and kept as a liquid. The mixes were amplified using inactivated Cytomegalovirus (CMV), Adenovirus (ADV) or Herpes simplex virus 2 (HSV2). Reactions were prepared in the presence of 30% human saliva, 30% Universal Transport Medium (UTM) + an artificial sputum swab and Viral Transport Medium (VTM). All reactions were incubated at 65 °C for 60 minutes, and average time to results (TTR) was measured at 1:10 of end fluorescence. Error bars represent the standard deviation across four technical replicates. Results show that MDX134 ( blue ) has a shorter TTR than NEB WarmStart® ( orange ) amplification across all three sample orange types. The data suggests that MDX134 performs better with faster amplification across the range of inhibitors.
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