Superior specificity using samples containing 15% sputum
Lyo-Ready™ Direct DNA LAMP Saliva (MDX134)
Amplification of Mycoplasma pneumoniae genomic DNA (200 copies) was compared using Lyo-Ready™ Direct DNA LAMP Saliva (MDX134) against NEB WarmStart® in samples containing 0% or 15% artificial sputum. Reactions were incubated at 65 °C for 60 minutes, and TTR was measured at 1:10 of end fluorescence. No NTCs were detected in this assay. Lyo-Ready™ Direct DNA LAMP Saliva (MDX134) with 0% ( light blue ) and 15% ( dark blue ) artificial sputum demonstrated stronger inhibitor tolerance, retaining its fast TTR in the presence of 15% artificial sputum. In contrast, NEB WarmStart ® with 0% artificial sputum ( orange ) amplified slower, as shown by a later TTR, than either reaction using Lyo-Ready™ Direct DNA LAMP Saliva. In addition, in samples containing 15% artificial sputum ( red ), NEB WarmStart® had a delayed amplification of 40 min and the longest TTR of all the reactions.
MDX134 0% Sputum | MDX134 15% Sputum | NEB WarmStart® 0% Sputum | NEB WarmStart® 15% Sputum
Maintains consistent performance in samples containing up to 60% saliva
Lyo-Ready™ Direct RNA/DNA LAMP Saliva (MDX135)
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Lyo-Ready™ Direct RNA/DNA LAMP Saliva (MDX135) was lyophilized with Mycobacterium tuberculosis (MTB) primers and resuspended with increasing amounts of human saliva containing M. tuberculosis (from 10% - 72%). Reactions were incubated at 65 °C for 60 minutes, and TTR was measured at 1:10 of end fluorescence. The results illustrate that Lyo- Ready™ Direct RNA/DNA LAMP Saliva maintains a robust performance up 60% saliva and demonstrates consistently late NTCs.
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