Specimen-specific ™ LAMP & RT-LAMP Mixes
Stool-specific Mixes
Stool
Diarrhoeal disease is the 8th leading cause of death globally and the 2nd leading cause of death in children under the age of five. It is caused by a variety of bacterial, viral and parasitic organisms and is usually spread through the fecal-oral route or by foodborne transmission. Stool has been used a diagnostic specimen to detect the presence of these organisms, and also to diagnose certain cancers and inform of overall gut health. In order to provide the right treatment and prevent serious complications, rapid identification of gastrointestinal infections
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL 800 Rxns 50 mL 8,000 Rxns 5 mL 800 Rxns 50 mL 8,000 Rxns
Lyo-Ready Direct DNA LAMP Stool Mix
MDX144
Lyo-Ready Direct RNA/DNA LAMP Stool Mix
MDX145
is crucial. This can be carried out using point-of-care (POC) diagnostic tests that are simple to use, involve minimal-to-no- sample pre-processing, do not require cold room storage and are as sensitive and specific as laboratory-based tests. Assays using stool as a specimen can pose a substantial challenge for POC assays as stool samples can be complex, containing food debris, desquamated epithelial cells and mucus, and potential LAMP inhibitors such as bile salts, polysaccharides, hematin and catabolic substances. Meridian has developed Lyo-Ready TM Direct DNA LAMP Stool and Lyo-Ready TM RNA/DNA LAMP Stool mixes to combine high inhibitor tolerance with fast, robust isothermal amplification to enable highly sensitive direct detection using crudely processed stool samples. The mixes contain excipients and an optimized buffer system that is compatible with lyophilization to create ambient-temperature stable assays which have more than two-year’s shelf-life; and are uniquely designed to overcome very high concentrations of inhibitors and so do not require a DNA or RNA purification step to purify a patient sample.
Superior Assay Sensitivity in Challenging Environments such as Low Copy Input and High Bile Salt Concentration
A) Lyo-Ready ™ Direct DNA LAMP Stool (MDX144)
B) Lyo-Ready ™ Direct RNA/DNA LAMP Stool (MDX145)
Respiratory Syncytial Virus
Salmonella typhimurium
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10
10
0
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1000
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10
1
MDX144 | NEB WarmStart ® | Thermo SuperScript ™ IV
MDX145 | NEB WarmStart ® | Thermo SuperScript ™ IV
A) Lyo-Ready ™ Direct DNA LAMP Stool (MDX144) liquid format ( blue ), was used for the amplification of 1,000, 100 and 10 and 1 copies of Salmonella DNA in the presence of 3 mg/mL bile salts and B) Lyo-Ready ™ Direct RNA/DNA LAMP Stool (MDX145) liquid format ( blue ) was used for the amplification of Respiratory Syncytial Virus (RSV) in the presence of 0 mg/mL, 3 mg/mL and 5 mg/mL bile salts. The results were compared using the same primers and DNA/RNA template for NEB WarmStart ® ( orange ) and Thermo SuperScript ( black ). Reactions were incubated at 65°C for 60 minutes, and TTR was measured at 1:10 of end fluorescence. Error bars represent the standard deviation across four technical replicates. The results demonstrate the high sensitivity of Lyo-Ready ™ Direct DNA LAMP Stool, even at 1 copy of DNA, and both mixes’ high tolerance to bile salt.
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