Sensitive Detection in Multiplex Assays in the Presence of Stool Inhibitors
Lyo-Ready™ Direct DNA qPCR Stool (MDX142)
Escherichia coli O157
Salmonella typhimurium
Campylobacter jejuni
Lyo-Ready ™ Direct DNA qPCR Stool | KAPA Probe Force | UltraPlex ™
E. coli O157 (Shiga-like/Verotoxin 2 gene), Salmonella typhimurium (16S-23S rRNA gene internal transcribed spacer) and Campylobacter jejuni (16S-23S rRNA gene internal transcribed spacer) were amplified in a triplex reaction using lyophilized Lyo-Ready ™ Direct DNA qPCR Stool ( red ) and universal mixes, KAPA Probe Force (Roche, green ) and UltraPlex ™ (QuantBio, grey ) in the presence of 6.6 mg/mL human stool. The results illustrate that Lyo-Ready ™ Direct DNA qPCR Stool is not affected by high concentrations of human stool, unlike the other mixes, allowing higher multiplexing capacity and greater reproducibility.
Lyo-Ready™ Direct RNA/DNA qPCR Stool (MDX143)
Rotavirus
Norovirus
Astrovirus
Campylobacter jejuni
Lyo-Ready ™ Direct RNA/DNA qPCR Stool | TaqPath ™ | UltraPlex ™
Three RNA targets (Rotavirus, Norovirus and Astrovirus) and one DNA target ( Campylobacter jejuni ) were amplified in a quadruplex reaction using lyophilized Lyo-Ready ™ Direct RNA/DNA qPCR Stool ( red ) and universal mixes, TaqPath ™ (Thermo, black ) and UltraPlex ™ (QuantBio, grey ) in the presence of 1.5 mg/mL bile salts. The results illustrate that unlike TaqPath ™ and Ultraplex ™ which are universal mixes, Lyo-Ready ™ Direct RNA/DNA qPCR Stool is less affected by high concentrations of bile salts, allowing higher multiplexing capacity and greater reproducibility.
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