Lyo-Ready™ Direct RNA/DNA qPCR Stool (MDX143)
Standard cycling
Fast cycling
Two mammalian RNA targets ( red ) were amplified using standard qPCR cycling conditions and the fastest cycling conditions available on a Quantstudio ™ 7 Flex Real-Time PCR System in the presence of 1.5 mg/mL human stool. The results illustrate the higher performance and end-fluorescence of Lyo-Ready ™ Direct RNA/DNA qPCR Stool (MDX143) in both standard (10 min reverse transcription step followed by 40 cycles of 10 sec @ 95ºC and 30 sec @ 60ºC) and fast (5 min reverse transcription step followed by 40 cycles of 1 sec @ 95ºC and 1 sec @ 60ºC) qPCR cycling conditions, without compromising the sensitivity or reproducibility of the assay.
Stable Shelf-life for 12+ Months in Lyophilized Format
A)
B)
Lyophilized | Liquid
Lyo-Ready Direct DNA qPCR Stool (MDX142) and Lyo-Ready Direct RNA/DNA qPCR Stool (MDX143) were lyophilized, and their stability was tested in an accelerated stability study. The lyophilized mixes ( red ) were incubated at 37ºC for 4 weeks and tested against freshly prepared liquid mixes ( blue ) in A) a qPCR assay with 20% artificial stool or B) an RT-qPCR assay with 5% artificial stool. The lyophilized mixes retained the same activity as the liquid mixes (<0.5 Δ Ct) over time. Results indicate that the lyophilized mixes have a projected stability of at least 12 months at ambient temperature.
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05/22
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