C) Hemin
Amplification of CYP3A4, a steriod hydroxalyase, was compared in the presence of 2 mM Hemin using Lyo-Ready TM Genotyping Direct qPCR Blood, Kapa Probe Force, TaqPath TM and Type-it Fast Kits.
A/
B/
2 mM Hemin was tested with A/ Lyo-Ready TM Genotyping Direct qPCR Blood, B/ Kapa Probe Force, C/ TaqPath TM and D/ Type-it Fast Kits using CYP3A4, a steroid hydroxylase. Homozygous allele A ( red ) and allele C ( blue ) and heterozygous allele A/C ( green ) with a NTC ( black ) and x for undetermined. Genotyping analysis from the Lyo-Ready TM Genotyping Direct qPCR Blood Mix illustrate accurate allelic discrimination clustering in the presence of Hemin.
C/
D/
Allele A | Allele C | Allele A/C | NTC
Superior Sensitivity and Reproducibility in Samples Containing 10,000 copies to 1 copy of DNA in 10% Plasma
Activity of Lyo-Ready TM Genotyping Direct qPCR Blood ( blue ) was compared to KAPA Probe Force ( green ), TaqPath TM ( black ), and Type-it Fast ( light blue ) kits in a qPCR assay, using a 10-fold serial dilution of VLP DNA (10,000, 1000, 100, 10 and 1 copies respectively), in the presence of 10% plasma. The results illustrate that Lyo-Ready TM Genotyping Direct qPCR Blood has significantly higher sensitivity and reproducibility in assays detecting high or low copies of DNA template and in the presence of PCR inhibitors, resulting in tighter, more discrete, well-defined cluster.
MDX128 | KAPA Probe Force | TaqPath ™ | Type-it Fast
www.meridianbioscience.com/lifescience
Powered by FlippingBook