Ability to Extend Shelf-life to Over 12 Months in Lyophilized Format
A) Amplification plot
Homozygous A/A
Heterozygous A/C
Homozygous C/C
B) End Fluorescence
C) Allelic discrimination
500,000
450,000
400,000
350,000
300,000
250,000
200,000
150,000
100,000
50,000
0
AA-FAM
AC-FAM AC-HEX
CC-HEX
Liquid | Lyophilized
Lyo-Ready TM Genotyping Direct qPCR Blood was lyophilized, and the stability of the dried assays ( red ) was tested after 1 month at 37°C against fresh liquid mix ( blue ) in the presence of 5% EDTA Blood (A/ and B/). The lyophilized mixes retained the same performance as the liquid mix (C/ homozygous allele A ( red ) and allele C ( blue ) and heterozygous allele A/C (green) with a NTC (black) and x for undetermined). Results suggest that Lyo-Ready TM Genotyping Direct qPCR Blood mix has a projected stability of more than 18 months at ambient temperature
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