High reaction efficiency with plasma, serum or whole blood samples containing anticoagulant K2-ETDA
Air-Dryable ™ Direct DNA qPCR Blood (MDX092)
Plasmid DNA containing the targets S. aureus, P. falciparum and Epstein Barr virus was spiked into 10% serum or 10% K2-EDTA plasma and amplified in a triplex reaction using air-dried MDX092 ( red ) (dried in a Memmert Universal oven UF260plus) and kits from KAPA Probe Force (Roche, green ) and TaqPath ™ (Thermo, black ). The results illustrate higher end florescence and better sensitivity with MDX092 than with the other mixes.
Staphylococcus aureus
Plasmodium falciparum
Epstein-Barr Virus
Air-dried | KAPA Probe Force (Roche) | TaqPath ™ (Thermo)
Air-Dryable ™ Direct RNA/DNA qPCR Blood (MDX121)
Dengue
S. pneumoniae
Zika
Chikungunya
Air-dried | Ultraplex ™ (QuantBio) | TaqPath ™ (Thermo)
Three viral RNA targets (Dengue, Zika and Chikungunya) and one DNA target ( S. pneumonia ) were amplified in a quadruplex reaction using air-dried MDX121 ( red ) and liquid mixes from Ultraplex ™ (QuantBio, grey ) and TaqPath ™ 1-Step (Thermo, black ) in the presence of 5% K2-EDTA blood or 5% K2-EDTA plasma. Air-Dryable ™ Direct RNA/DNA qPCR Blood has higher multiplexing capacity than the other mixes.
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