Air-Dryable ™ Direct RNA/DNA qPCR Blood (MDX121)
5% K2-EDTA whole blood
Activity of air-dried Air-Dryable Direct RNA/DNA qPCR Blood (MDX121) in both air-dried ( red ) format and liquid format ( blue ) were compared to Ultraplex ™ (QuantBio, grey ) in a multiplexing qPCR assay, using a 10-fold serial dilution of mammalian RNA (10,000, 1000, 100 and 10 copies respectively), in the presence of 5% K2-EDTA whole blood. The results illustrate that the Air-Dryable Direct RNA/DNA qPCR Blood retains the ability to efficiently amplified and shows higher end florescence and sensitivity than the supplier Q mix.
Air-dried | Liquid | Ultraplex™ (QuantBio)
Air-dried mixes maintain their shelf-life for up to 12 months A) Air-Dryable ™ Direct DNA qPCR Blood (MDX092)
B) Air-Dryable ™ Direct RNA/DNA qPCR Blood (MDX121)
Air-dried | Liquid
The Air-Dryable Direct Blood mixes were air dried and their stability was tested in an accelerated stability study. (A) Three DNA targets were amplified with Air-Dryable Direct DNA qPCR Blood (MDX092) and (B) Three mammalian targets were amplified with Air-Dryable Direct RNA/DNA qPCR Blood (MDX121) that was air-dried ( red ) and incubated a 37 °C for 1 month and tested against the fresh liquid mix ( blue ) in assays with 5% K2-EDTA whole blood. Results suggest that the air-dried mixes are active following accelerated stability tests with projected 12 months stability at ambient temperature.
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