RT-qPCR Master Mixes
Air-Dryable TM 1-Step RT-qPCR Mix PRODUCT HIGHLIGHTS • Simple and easy to use • Inhibitor-tolerant mix designed singleplex or multiplex detection from crude lysates • Can be used in a wet or dry format, and compatible with a range of air-drying protocols to produce an ambient temperature stable mix • Compatible with a range of air-drying protocols to produce an ambient temperature stable mix High-tolerance to PCR inhibitors in sputum samples Amplification profile of a mouse RNA target spiked into samples containing 10%, 5% or 0% artificial sputum in Universal Transport Media (UTM). The data illustrates that the performance of Air-Dryable 1-Step RT-qPCR Mix (MDX095) exhibits high tolerance towards inhibitors present in artificial sputum and UTM. Air-Dryable TM RNA/DNA LAMP Mix PRODUCT HIGHLIGHTS • Fast reaction kinetics – results in half the time • Complete, pre-formulated mix for isothermal amplification of DNA and RNA targets – just add custom primers and probes • Ideal for POC diagnostic platforms or automated high-throughput instruments
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL
1,000 Rxns
Air-Dryable ™ 1-Step RT-qPCR Mix
MDX095
50 mL
10,000 Rxns
0% UTM+Sputum 5% UTM+Sputum 10% UTM+Sputum
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL
800 Rxns
Air-Dryable ™ RNA/DNA LAMP Mix
MDX118
50 mL
8,000 Rxns
• Concentrated 4x formulation with flexibility in final assay format (liquid or air-dried) and then can you do the same adjustments to title
High reaction specificity with little-to-no non-specific amplification Air-Dryable ™ RNA/DNA LAMP (MDX118) ( blue ) was compared to NEB WarmStart ® ( orange ) for detection of EBV DNA (1000 ipc) and MS2 RNA (1000 ipc). Reactions were incubated at 65°C for 60 min and TTR were measured at 1:10 of end fluorescence. Amplification plots of 3 technical replicates show Air-Dryable ™ RNA/DNA LAMP mix has earlier TTR and no non-specific amplification ( ) compared to NEB WarmStart ® which has detectable amplification for non-template controls (NTC) ( ).
EBV-DNA
MS2-RNA
MDX118 | NEB WarmStart ®
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