Bacterial and Fungal DNA Amplification

Bacterial and Fungal DNA Amplification DNA Polymerases and Optimized Master mix

Suitable applications

Food Testing

Water Testing

Environmental

Human Diagnostics

Vet Health

DNA Barcoding

PCR amplification for the detection of fungal and bacterial nucleic acids offers greater sensitivity over current culture-based methods, the potential for multiplex analysis, and can be applied to a variety of specimen types. However, trace amounts of residual bacterial and fungal DNA can be found in commercially available PCR reagents which can cause contamination and non-specific amplification. The most effective strategy to reduce false-positive reactions due to residual DNA contamination is to use reagents with low DNA content and low bioburden.

DNA Polymerases

Low DNA Taq • Heat-activated thermostable DNA polymerase • Convenient room-temperature reaction set-up • Ideal for bacterial and fungal detection assays • Proven performance in commercial high- throughput and high-multiplex assays

PRODUCT

CAT NO.

VOLUME REACTIONS

100 µL

500 Units

Low DNA Taq HS 5 U/µL

MDX009

10 mL

50,000 Units

50 µL

500 Units

Low DNA Taq HS 10 U/µL

MDX010

5 mL

50,000 Units

www.MeridianLifeScience.com

Optimized qPCR Master Mix

Low DNA qPCR Mix • 2x Mastermix containing a heat-activated thermostable DNA polymerase and optimized buffer • Robust performance and high tolerance to common PCR inhibitors • Extended stability at ambient temperature • Ideal for high-multiplex reactions

PRODUCT

CAT NO.

VOLUME REACTIONS

5 mL

500 Rxn

Low DNA qPCR Mix

MDX030

100 mL

10,000 Rxn

General Reagents

PRODUCT

CAT NO.

VOLUME

REACTIONS

qPCR controls Internal control designed to closely mimic test samples. Can be used to validate the extraction step and monitor any co-purification of PCR inhibitors. Controls are available with different dyes to fit with existing protocols.

qPCR Extraction Control RED (Quasar ® 670)

MDX026

10 mL

2,000 Rxn

qPCR Extraction Control ORANGE (Cal Fluor ® 560)

MDX027

10 mL

2,000 Rxn

General Reagents Uracil DNA Glycosylase (UDG) Enzyme that efficiently hydrolyzes uracil from ssDNA or dsDNA. Endonuclease, exonuclease, nickase and RNase-free Proteinase K Solution RNase and DNase free, ideal for removing endogenous nucleases when purifying native DNA or RNA RNase Inhibitor Inhibits a broad spectrum of eukaryotic RNases, including RNases A, B and C to control for contaminants in RT-PCR assays Tissue Extract-PCR Buffers Lysis and neutralization buffers optimized for use with Taq HS DNA Polymerase (Cat# MDX008) to perform PCR direct from crude lysate

MDX054

10 mL

10,000 Units

MDX055

25 mL

500 mg

250 µL

10,000 Units

MDX056

2.5 mL

100,000 Units

MDX004

1 Kit

1,000 Rxn

Ultra-pure (>99% determined by HPLC) dNTPs supplied as lithium salts are also available. They are sold individually (100mM), in sets (100mM) or as mixes (40mM and 100mM).

Ordering information: USA E: info@meridianlifescience.com Toll free: +1 800 327 6299 UK E: info.uk@meridianlifescience.com Tel: +44 (0)20 8830 5300

Germany E: info.de@meridianlifescience.com Tel: +49 (0)3371 60222 00 France E: info.fr@meridianlifescience.com Tel: +33 (0)1 42 56 04 40

美国迈迪安生命科学公司 电子邮件: vivian.li@meridianlifescience.com 电话: +86-159-1103-0750

Connect with us:

Australia E: info.au@meridianlifescience.com Tel: +61 (0)2 9209 4180

www.MeridianLifeScience.com

07/19

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