DNA Polymerases and Optimized Master mix. PCR amplification for the detection of fungal and bacterial nucleic acids offers greater sensitivity over current culture-based methods, the potential for multiplex analysis, and can be applied to a variety of specimen types. However, trace amounts of residual bacterial and fungal DNA can be found in commercially available PCR reagents which can cause contamination and non-specific amplification.
Bacterial and Fungal DNA Amplification DNA Polymerases and Optimized Master mix
PCR amplification for the detection of fungal and bacterial nucleic acids offers greater sensitivity over current culture-based methods, the potential for multiplex analysis, and can be applied to a variety of specimen types. However, trace amounts of residual bacterial and fungal DNA can be found in commercially available PCR reagents which can cause contamination and non-specific amplification. The most effective strategy to reduce false-positive reactions due to residual DNA contamination is to use reagents with low DNA content and low bioburden.
Low DNA Taq • Heat-activated thermostable DNA polymerase • Convenient room-temperature reaction set-up • Ideal for bacterial and fungal detection assays • Proven performance in commercial high- throughput and high-multiplex assays
Low DNA Taq HS 5 U/µL
Low DNA Taq HS 10 U/µL
Optimized qPCR Master Mix
Low DNA qPCR Mix • 2x Mastermix containing a heat-activated thermostable DNA polymerase and optimized buffer • Robust performance and high tolerance to common PCR inhibitors • Extended stability at ambient temperature • Ideal for high-multiplex reactions
Low DNA qPCR Mix
qPCR controls Internal control designed to closely mimic test samples. Can be used to validate the extraction step and monitor any co-purification of PCR inhibitors. Controls are available with different dyes to fit with existing protocols.
qPCR Extraction Control RED (Quasar ® 670)
qPCR Extraction Control ORANGE (Cal Fluor ® 560)
General Reagents Uracil DNA Glycosylase (UDG) Enzyme that efficiently hydrolyzes uracil from ssDNA or dsDNA. Endonuclease, exonuclease, nickase and RNase-free Proteinase K Solution RNase and DNase free, ideal for removing endogenous nucleases when purifying native DNA or RNA RNase Inhibitor Inhibits a broad spectrum of eukaryotic RNases, including RNases A, B and C to control for contaminants in RT-PCR assays Tissue Extract-PCR Buffers Lysis and neutralization buffers optimized for use with Taq HS DNA Polymerase (Cat# MDX008) to perform PCR direct from crude lysate
Ultra-pure (>99% determined by HPLC) dNTPs supplied as lithium salts are also available. They are sold individually (100mM), in sets (100mM) or as mixes (40mM and 100mM).
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