DNA Polymerases and Optimized Master mix. PCR amplification for the detection of fungal and bacterial nucleic acids offers greater sensitivity over current culture-based methods, the potential for multiplex analysis, and can be applied to a variety of specimen types. However, trace amounts of residual bacterial and fungal DNA can be found in commercially available PCR reagents which can cause contamination and non-specific amplification.
Bacterial and Fungal DNA Amplification DNA Polymerases and Optimized Master mix
Suitable applications
Food Testing
Water Testing
Environmental
Human Diagnostics
Vet Health
DNA Barcoding
PCR amplification for the detection of fungal and bacterial nucleic acids offers greater sensitivity over current culture-based methods, the potential for multiplex analysis, and can be applied to a variety of specimen types. However, trace amounts of residual bacterial and fungal DNA can be found in commercially available PCR reagents which can cause contamination and non-specific amplification. The most effective strategy to reduce false-positive reactions due to residual DNA contamination is to use reagents with low DNA content and low bioburden.
DNA Polymerases
Low DNA Taq • Heat-activated thermostable DNA polymerase • Convenient room-temperature reaction set-up • Ideal for bacterial and fungal detection assays • Proven performance in commercial high- throughput and high-multiplex assays
PRODUCT
CAT NO.
VOLUME REACTIONS
100 µL
500 Units
Low DNA Taq HS 5 U/µL
MDX009
10 mL
50,000 Units
50 µL
500 Units
Low DNA Taq HS 10 U/µL
MDX010
5 mL
50,000 Units
www.MeridianLifeScience.com
Optimized qPCR Master Mix
Low DNA qPCR Mix • 2x Mastermix containing a heat-activated thermostable DNA polymerase and optimized buffer • Robust performance and high tolerance to common PCR inhibitors • Extended stability at ambient temperature • Ideal for high-multiplex reactions
PRODUCT
CAT NO.
VOLUME REACTIONS
5 mL
500 Rxn
Low DNA qPCR Mix
MDX030
100 mL
10,000 Rxn
General Reagents
PRODUCT
CAT NO.
VOLUME
REACTIONS
qPCR controls Internal control designed to closely mimic test samples. Can be used to validate the extraction step and monitor any co-purification of PCR inhibitors. Controls are available with different dyes to fit with existing protocols.
qPCR Extraction Control RED (Quasar ® 670)
MDX026
10 mL
2,000 Rxn
qPCR Extraction Control ORANGE (Cal Fluor ® 560)
MDX027
10 mL
2,000 Rxn
General Reagents Uracil DNA Glycosylase (UDG) Enzyme that efficiently hydrolyzes uracil from ssDNA or dsDNA. Endonuclease, exonuclease, nickase and RNase-free Proteinase K Solution RNase and DNase free, ideal for removing endogenous nucleases when purifying native DNA or RNA RNase Inhibitor Inhibits a broad spectrum of eukaryotic RNases, including RNases A, B and C to control for contaminants in RT-PCR assays Tissue Extract-PCR Buffers Lysis and neutralization buffers optimized for use with Taq HS DNA Polymerase (Cat# MDX008) to perform PCR direct from crude lysate
MDX054
10 mL
10,000 Units
MDX055
25 mL
500 mg
250 µL
10,000 Units
MDX056
2.5 mL
100,000 Units
MDX004
1 Kit
1,000 Rxn
Ultra-pure (>99% determined by HPLC) dNTPs supplied as lithium salts are also available. They are sold individually (100mM), in sets (100mM) or as mixes (40mM and 100mM).
Ordering information: USA E: info@meridianlifescience.com Toll free: +1 800 327 6299 UK E: info.uk@meridianlifescience.com Tel: +44 (0)20 8830 5300
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www.MeridianLifeScience.com
07/19
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