Bst DNA Polymerase

High Performance Buffer

Inhibitor-Tolerant Bst Buffer ( MDX019 )

Data Highlights

Efficient amplification over different GC content and in the presence of sputum LAMP amplification of B. pertussis (57% GC), S. aureus (33% GC), Epstein-Barr Virus (59% GC) and M. tuberculosis (65% GC) in the presence of 20% artificial sputum medium. Inhibitor- Tolerant Bst Buffer (MDX019, dark orange) shows faster time-to-result (TTR) compared to a Standard Bst Buffer (blue) .

High reaction efficiency in the presence of increasing concentrations of saliva LAMP amplification of hBRCA1 gene from human genomic DNA spiked into human saliva with Inhibitor-Tolerant Bst Buffer (MDX019, dark orange) and Standard Bst Buffer (blue) . Inhibitor-Tolerant Bst Buffer shows no loss of activity with increasing concentrations of saliva.

Saliva (0-30%) final reaction concentration

Respiratory pathogen detection in presence of sputum

1 2 3 4 5 6 7 8 9 10

B. pertussis

S. aureus

Epstein Barr Virus

M. Tuberculosis

10% 20% 30%

Inhibitor-Tolerant Bst Buffer

Standard Bst Buffer

Inhibitor-Tolerant Bst Buffer

Standard Bst Buffer

Improved tolerance to Universal Transport Medium (UTM)

T. vaginalis DNA was spiked into UTM and added at final concentrations of 5%, 20% or 40% to reactions containing Inhibitor-Tolerant BST Buffer (MDX019, dark orange) or a Standard Bst Buffer (blue) . Reactions with Inhibitor-Tolerant Bst Buffer demonstrated better activity at high concentrations of UTM compared to reactions using only a Standard Buffer.

5% UTM

20% UTM

40% UTM

Higher reaction specificity LAMP amplification of 100 input copies of DNA template from B. pertussis, S. aureus , Epstein-Barr Virus and M. tuberculosis , along with non-template controls (NTC) using Inhibitor-Tolerant Bst Buffer (MDX019, dark orange) or a Standard Bst Buffer (blue) . Reactions containing Inhibitor-Tolerant Bst Buffer showed no NTC amplification (brown) or delayed amplification compared to reactions containing a Standard Bst Buffer.