Bst Reaction Buffer, 10x ( MDX076 )
Data Highlights
Exhibits High Tolerance to Salt Inhibition LAMP reactions were performed using Bst Reaction Buffer/Bst Polymerase (MDX076/MDX012, orange) or a competitor Bst Polymerase and buffer (gray) using increasing amounts of salt (potassium chloride). The time to results (TTR) illustrate that Bst Reaction Buffer performs well in the presence of high salt concentrations which can inhibit LAMP reactions.
Bst DNA Polymerase
Standard Bst Buffer
Enables Fast Polymerization LAMP reactions were performed using Meridian’s Bst Reaction Buffer/Bst DNA Polymerase (MDX076/MDX012, orange) and compared against three competing Bst enzymes/buffers [Supplier A (blue) , Supplier B (green) and Supplier C (purple) ]. Meridian’s Bst buffer/enzyme consistently reached the fastest reaction threshold times across three different targets (BRCA1 tumor suppressor gene, CFTR and S. stercoralis ).
A/ BRCA1 tumour suppressor gene
B/ Cystic Fibrosis Transmembrane Receptor (CFTR)
C/ Strongyloides stercoralis (Human pathogenic parasite)
Time [60 s interval]
Time [60 s interval]
Time [60 s interval]
High Stability Under High Temperature or Repeated Freeze/Thaw Bst Reaction Buffer/Bst DNA Polymerase (MDX076/MDX012) was left at (A) +4 ° C, ambient (room temperature) or +37 ° C for 4 hours, 24 hours or 1 week and (B) subjected to 5, 20 and 30 rounds of fast freeze/thawing, between -80 ° C and 0 ° C. The results illustrate that the Bst Mix MDX076/MDX012 is very stable, and able to withstand temperatures up to +37 ° C for a 1 week, or up to 30 freeze/thaw cycles without losing activity. Similar results were obtained with Bst Standard Buffer/ High-Conc. Glycerol-Free Bst Polymerase (MDX076/MDX018) under the same testing conditions. Data is available on request.
B/
A/
12.0 11.0 10.0
12 11 10
9.0 8.0 7.0 6.0 5.0 4.0 3.0 2.0 1.0 0.0
9 8 7 6 5 4 3 2 1 0
+4 o C
Ambient Storage Temperature
+37 o C
5
20 Freeze-thaw Cycles
30
Ordering information: USA 5171 Wilfong Road
Email: info@meridianlifescience.com Orders: orders@meridianlifescience.com www.MeridianLifeScience.com
Memphis, Tennessee 38134 Phone: +1 901-382-8716 Fax: +1 901-333-8223
Connect with us:
ISO 13485 Certified
10/21
Powered by FlippingBook