Inhibitor-Tolerant qPCR Mix
• Ready-to-use qPCR mix engineered with a proprietary buffer system designed for overcoming common PCR inhibitors • Allows for DNA amplification direct from crude lysate or unprocessed samples such as urine, cerebrospinal fluid (CSF), milk and blood • Eliminates need for sample extraction and speeds up assay TAT • Lyo-ready master mix can be lyophilized, just add primers and probe to create an ambient temperature stable assay
PRODUCT
CAT NO.
VOLUME REACTIONS
5 mL 500 Rxns 100 mL 10,000 Rxns 500 Rxns 40 mL 10,000 Rxns 2 mL 5 mL 500 Rxns 50 mL 10,000 Rxns
Inhibitor-Tolerant qPCR Mix
MDX013
Inhibitor-Tolerant qPCR Mix, 5x
MDX073
Lyo-Ready Inhibitor- Tolerant qPCR Mix 4x
MDX184
High qPCR efficiency across a range of inhibitors
Reaction efficiencies were determined from reactions containing a variety of known PCR inhibitors ranging from whole blood to biofluids (20% in reaction). The results demonstrate that the reaction efficiency of the Inhibitor-Tolerant qPCR Mix (MDX013) remained within 90-110% in the presence of a wide range of common PCR inhibitors.
WHOLE BLOOD SPECIMENS
Amplification Plot A
Amplification Plot B
40.0
32.0 34.0 36.0 38.0
30.0
28.0
Amplification Plot C
26.0
Direct detection of (A) 25% (B) 46% and (C) 64% GC-rich DNA from 20% whole blood using MDX013 (green) and mixes from suppliers R (red) and T (blue).
20%
10%
1%
Percent Blood
Direct detection of GC-rich (64%) DNA from 1% to 20% whole blood using MDX013 (green) and mixes from other suppliers.
SPINAL FLUID, URINE AND MILK SPECIMENS
Amplification Plot
Amplification Plot
Amplification Plot
Cerebrospinal fluid
Urine
Cow milk
A 10-fold serial dilution of genomic DNA was spiked into cerebrospinal fluid, human urine or cow whole milk and the DNA was amplified, using MDX013 (green) and supplier R (red). The results illustrate that MDX013 is more sensitive than supplier R mix, as lower dilutions could be detected with better efficiencies, across all three sample types.
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