SPINAL FLUID, URINE AND MILK SPECIMENS
Cerebrospinal fluid
Urine
Cow milk
A 10-fold serial dilution of genomic DNA was spiked into cerebrospinal fluid, human urine or cow whole milk and the DNA was amplified, using MDX013 ( green ) and supplier R ( red ). The results illustrate that MDX013 is more sensitive than supplier R mix, as lower dilutions could be detected with better efficiencies, across all three sample types.
Inhibitor-Tolerant RT-qPCR Mix • Designed for amplification from crude samples without the need for complex or time-consuming extraction • 4x concentration master mix allows for greater sample volume • Single use one-step mix. Only add primers, probes and clinical samples • Suitable for single and multiplex detection of RNA and DNA pathogens
PRODUCT
CAT NO.
VOLUME REACTIONS
5 mL
1,000 Rxn
Inhibitor-Tolerant RT-qPCR Mix
MDX016
50 mL
10,000 Rxn
Inhibitor-Tolerant RT-qPCR Mix exhibits a high tolerance to PCR inhibitors from clinical samples
SALIVA SPECIMENS
10% Saliva
20% Saliva
MDX016 ( red ) and MDX032 ( black ) amplification traces of Influenza A spike in presence of saliva swabs (COPAN ESwab 359C) at 10% (left) and 20% (right) final concentration. With earlier Ct (approx. 4 Ct) and higher fluorescence (approx. +50%), the results demonstrate the superiority of Inhibitor-Tolerant RT-qPCR Mix (MDX016) against a standard RT-qPCR Mix (MDX032) for the detection of viral RNA in presence of saliva swab resuspension in UTM.
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