Inhibitor-Tolerant qPCR Mix
• Ready-to-use qPCR mix engineered with a proprietary buffer system designed for overcoming common PCR inhibitors • Allows for DNA amplification direct from crude lysate or unprocessed samples such as urine, cerebral spinal fluid (CSF), milk and blood
PRODUCT
CAT NO.
VOLUME REACTIONS
5 mL
500 Rxn
Inhibitor-Tolerant qPCR Mix
MDX013
100 mL
10,000 Rxn
• Ideal for developing qualitative assays where sample is limited • Eliminates need for sample extraction and speeds up assay TAT
High qPCR efficiency across a range of inhibitors
Inhibitor-Tolerant qPCR Mix Reaction Efficiencies
100
Reaction efficiencies were determined from reactions containing a variety of known PCR inhibitors ranging from whole blood to biofluids (20% in reaction). The results demonstrate that the reaction efficiency of the Inhibitor- Tolerant qPCR Mix (MDX013) remained within 90-110% in the presence of a wide range of common PCR inhibitors.
80
60
40
20
0
Efficient amplification from various liquid sample types (spinal fluid, urine, milk and blood).
WHOLE BLOOD SPECIMENS
40.0 32.0 34.0 36.0 38.0 30.0 28.0 26.0
A
B
Direct detection of (A) 25% (B) 46% and (C) 64% GC-rich DNA from 20% whole blood using MDX013 ( green ) and mixes from suppliers R ( red ) and T ( blue ).
C
20% 10% 1%
Percent Blood
Direct detection of GC-rich (64%) DNA from 1% to 20% whole blood using MDX013 ( green ) and mixes from other suppliers.
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