Inhibitor-Tolerant qPCR & RT-qPCR Mixes 0525

SPUTUM SPECIMENS

Amplification profile of inactivated influenza virus spiked into samples containing 5% sputum or no sputum. The data illustrates that the performance of Inhibitor-Tolerant RT-qPCR Mix (MDX016) in the presence of 5% artificial sputum (red) is the same as the RT-qPCR Mix RT-qPCR Mix with no sputum (blue). In contrast, the sensitivity and performance of the standard RT-qPCR Mix significantly decreases in the presence of 5% artificial sputum (black) compared to no sputum or compared to MDX016 with sputum.

1.25% Sputum

2.5% Sputum

5% Sputum

Amplification profiles of inactivated influenza virus spiked into samples containing various concentrations of sputum (1.25% – 5%). Results demonstrate that MDX016 (red) exhibits a higher tolerance to inhibitors present in artificial sputum (Kirchner, S. et al . J Vis Exp (64) 2012) compared to the standard RT-qPCR Mix (black).

STOOL SPECIMENS

Adenovirus

Norovirus GII

Campylobacter jejuni

Rotavirus A

Amplification profiles of 4 pathogens (RNA: Norovirus and Rotavirus, DNA: Adenovirus, C. jejuni ) in a multiplex reaction containing 5% (yellow), 10% (red) and 20% (black) stool extract. The results demonstrate the multiplexing capability of MDX016 in the presence of inhibitors found in stool (up to 20% final volume).

Ordering information: USA 5171 Wilfong Road Memphis, Tennessee 38134 Phone: +1 901-382-8716 Fax: +1 901-333-8223

Email: info@meridianlifescience.com Orders: orders@meridianlifescience.com www.MeridianBioscience.com/lifescience

Connect with us:

ISO 13485 Certified

05/25

Powered by