SPUTUM SPECIMENS
Amplification profile of inactivated influenza virus spiked into samples containing 5% sputum or no sputum. The data illustrates that the performance of Inhibitor-Tolerant RT-qPCR Mix (MDX016) in the presence of 5% artificial sputum (red) is the same as the RT-qPCR Mix RT-qPCR Mix with no sputum (blue). In contrast, the sensitivity and performance of the standard RT-qPCR Mix significantly decreases in the presence of 5% artificial sputum (black) compared to no sputum or compared to MDX016 with sputum.
1.25% Sputum
2.5% Sputum
5% Sputum
Amplification profiles of inactivated influenza virus spiked into samples containing various concentrations of sputum (1.25% – 5%). Results demonstrate that MDX016 (red) exhibits a higher tolerance to inhibitors present in artificial sputum (Kirchner, S. et al . J Vis Exp (64) 2012) compared to the standard RT-qPCR Mix (black).
STOOL SPECIMENS
Adenovirus
Norovirus GII
Campylobacter jejuni
Rotavirus A
Amplification profiles of 4 pathogens (RNA: Norovirus and Rotavirus, DNA: Adenovirus, C. jejuni ) in a multiplex reaction containing 5% (yellow), 10% (red) and 20% (black) stool extract. The results demonstrate the multiplexing capability of MDX016 in the presence of inhibitors found in stool (up to 20% final volume).
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