Amplification profile of inactivated influenza virus spiked into samples containing 5% sputum or no sputum. The data illustrates that the performance of Inhibitor-Tolerant RT-qPCR Mix (MDX016) in the presence of 5% artificial sputum ( red ) is the same Fast One-Step RT-qPCR Mix (MDX032) with no sputum ( blue ). In contrast, the sensitivity and performance of MDX032 significantly decreases in the presence of 5% artificial sputum ( black ) compared to no sputum or compared to MDX016 with sputum.
Amplification profiles of inactivated influenza virus spiked into samples containing various concentrations of sputum (1.25% - 5%). Results demonstrate that MDX016 ( red ) exhibits a higher tolerance to inhibitors present in artificial sputum (Kirchner, S. et al. J Vis Exp (64) 2012) compared to the standard MDX032 ( black ).
Amplification profiles of 4 pathogens (RNA: Norovirus and Rotavirus, DNA: Adenovirus, C. jejuni ) in a multiplex reaction containing 5% ( yellow ), 10% ( red ) and 20% ( black ) stool extract. The results demonstrate the multiplexing capability of MDX016 in the presence of inhibitors found in stool (up to 20% final volume).
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