Universal LAMP & RT-LAMP Mixes
PRODUCT
CAT. NO.
VOLUME REACTIONS
Superior Sensitivity with Shorter Time-to-Results (TTR) A MDX119 | Air-Dryable TM DNA LAMP Mix
5 mL
800
B MDX118 | Air-Dryable TM RNA/DNA LAMP Mix
Air-Dryable TM LAMP Mix
MDX119
50 mL
8,000
80
50
40
5 mL
800
60
Air-Dryable TM RT-LAMP Mix
MDX118
30
50 mL
8,000
40
20
5 mL
800
20
Lyo-Ready LAMP Mix
MDX097
10
50 mL
8,000
0
0
1000 copies/rxn 100 copies/rxn 20 copies/rxn
NTC
1000 copies/rxn 500 copies/rxn 100 copies/rxn 10 copies/rxn
5 mL
800
(A) Conserved regions from the S and P genes of Hepatitis B Virus (1,000, 100 and 20 copies respectively) were amplified using Air-Dryable TM DNA LAMP ( MDX119 ) in an air-dried ( red ) or liquid ( blue ) format or using NEB WarmStart ® ( green ). (B) Emesvirus (MS2) was amplified using AirDryable TM RNA/DNA LAMP ( MDX118 ) in an air-dried ( red ) or liquid ( blue ) format or using NEB WarmStart ® ( green ). Reactions were incubated at 65°C for 60 min and TTR were measured at 1:10 of end fluorescence. Error bars represent standard deviation over 4 technical replicates. At lower copy numbers, NEB WarmStart ® (green) exhibited significantly lower reaction reproducibility compared to Air-Dryable TM DNA LAMP Mix, and a lower TTR was observed for reactions using both Air-Dryable TM DNA LAMP Mix and Air-Dryable TM DNA/RNA LAMP Mix across all dilutions of the Hepatitis B gene and MS2-RNA respectively. The results therefore demonstrate greater sensitivity of Air-Dryable TM LAMP Mixes with shorter TTR across all template concentrations. MDX119/118 (Air-Dried) | MDX119/118 (Liquid) | NEB WarmStart ®
Lyo-Ready RT-LAMP 1-Step Mix
MDX108
50 mL
8,000
Meridian’s Lyo-Ready and Air-Dryable TM LAMP and RT-LAMP Master Mixes are the market-leading solution for molecular diagnostic assays requiring fast cycling, superior sensitivity, high inhibitor tolerance and ambient temperature stability. They are 4x concentrated, glycerol-free and contain specialized excipients that are compatible with lyophilization or air-drying to create ambient-temperature stable assays, or they can be used as a wet mix. They are highly inhibitor-tolerant and ultra-sensitive making them ideal for point-of-care or machine-automated multiplexing assays. As universal mixes, they are highly resistant to various qPCR inhibitors and can be used across a range of patient sample types.
PRODUCT
CAT. NO.
VOLUME REACTIONS
5 mL
800
Fast Polymerization for Quick Sample-to-Answer Time MDX097 | Lyo-Ready LAMP Mix
Lyo-Ready AOF dUTP DNA LAMP
MDX301
50 mL
8,000
20
The average time-to-result (TTR) for Lyo-Ready LAMP Mix ( MDX097 - teal green ) and NEB WarmStart ® ( light green ) were compared for three genes, 9TV10 from Trichomonas vaginalis (100 copies), hBRCA1 from human genomic DNA (200 copies) and MTB1 from Mycobacterium tuberculosis (1,000 copies). The results demonstrate the faster speed of Lyo-Ready LAMP Mix, with earlier TTR values when compared to NEB WarmStart ® . Reactions were incubated at 65°C for 60 min and TTR were measured at 1:10 of end fluorescence.
5 mL
800
Lyo-Ready AOF dUTP RNA/DNA LAMP
MDX302
50 mL 8,000 Meridian’s Lyo-Ready AOF dUTP DNA LAMP and RT-LAMP Master Mixes are also designed to prevent carryover contamination and minimize false positives, making them ideal for high-throughput and multiplex assays, and the BSA-free formulation reduces reliance on animal-sourced reagents while enhancing efficiency and consistency. Faster Time-to-Result (TTR) and Better Reproducibility A MDX301 | Lyo-Ready AOF dUTP DNA LAMP B MDX302 | Lyo-Ready AOF dUTP RNA/DNA LAMP
15
10
5
0
9TV10 - T. Vaginalis
hBRCA-1M - Human DNA MTB1 - M. tuberculosis
MDX097 | NEB WarmStart ®
30
30
Fast Polymerization for Quick Sample-to-Answer Time MDX118 | Air-Dryable TM RNA/DNA LAMP Mix
25
25
20
20
Air-Dryable TM RNA/DNA LAMP ( MDX118 - teal green ) was compared to NEB WarmStart ® ( light green ) for detection of EBV DNA (1,000 ipc) and MS2 RNA (1,000 ipc). Reactions were incubated at 65°C for 60 min and TTR were measured at 1:10 of end fluorescence. Amplification plots of 3 technical replicates demon- strate AirDryable TM RNA/DNA LAMP Mix has earlier TTR and no non-specific amplification compared to NEB WarmStart ® , which has detectable amplification for non-template controls (NTC).
15
15
10
10
5
5
0
0
BRCA1
M. pneumoniae
T. vaginalis
Hepatitis C
West Nile Virus
Chikungunya
(A) Lyo-Ready AOF dUTP DNA LAMP ( MDX301 - teal green ) was amplified with BRCA1 (1,000 copies), M. pneumoniae (100 copies) and T. vaginalis (100 copies), and (B) Lyo-Ready AOF dUTP RNA/DNA LAMP ( MDX302 - teal green ) was amplified with Hepatitis C Virus (100 copies), West Nile Virus (100 copies) and Chikungunya (1,000 copies). They were compared to the same samples amplified with NEB WarmStart ® Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG) ( light green ). The results demonstrate the faster speed (time to results) and better reproducibility (shorter error bars) of the Lyo-Ready AOF dUTP LAMP mixes compared to other suppliers.
MDX118 | NEB WarmStart ®
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