Over the past decade, loop-mediated isothermal amplification (LAMP) technology has played an increasingly important role in point-of-care (POC) diagnostics. It offers several advantages over qPCR and other amplification techniques in terms of sample-to-answer time, sensitivity, specificity, cost, robustness, and compatibility with battery-powered devices, making it ideal for field-deployable diagnostics in resource-limited settings. LAMP-based assays have been used for numerous applications, including the detection of pathogens such as Salmonella and malaria, genetically modified crop contamination and in forensics to specifically detect human DNA.
Lyo-Ready & Air-Dryable TM LAMP & RT-LAMP Master Mixes
LAMP offers several advantages over qPCR and other amplification techniques in terms of sample-to- answer time, sensitivity, specificity, cost and robust amplification at a constant temperature. It is also simpler to operate and uses less expensive, portable equipment, making it ideal for field deployable diagnostics in resource-limited settings. LAMP based assays have been used for numerous applications including the detection of pathogens such as salmonella and malaria, genetically modified crop contamination and in forensics to specifically detect human DNA. In addition, RT-LAMP is widely used for screening for a number of pathogens, particularly RNA viruses, such as influenza, rotavirus, hepatitis C, West Nile fever, Dengue virus, Ebola virus and most recently, SARS-CoV-2. Ambient-temperature stability is particularly important in POC diagnostics. It reduces the cold-chain requirements for shipping and storage, extends the assay shelf-life, and increases the portability of the assay making it accessible to remote regions with limited resources. Meridian’s Lyo-Ready and Air-Dryable TM technologies simplify the development of ambient-temperature stable, multiplex molecular assays. They are 4x concentrated, glycerol-free and contain specialized excipients that are compatible with lyophilization or air-drying, requiring no further optimization besides the addition of primers and probes. Over the past decade, isothermal amplification techniques such as hybridized chain reaction (HCA), multiple displacement amplification (MDA) and loop-mediated isothermal amplification (LAMP) have played an increasingly important role in point-of-care (POC) diagnostics.
PRODUCT FEATURES
• Ideal for POC diagnostic platforms or automated high-throughput instruments • Available in universal or Specimen-specific TM formulations for blood, saliva, urine or stool and suitable for direct detection assays using crude samples • Fully optimized, concentrated 4x master mixes — just add primers and probes • Contains all the required excipients for subsequent lyophilization or air-drying • Shelf life greater than 12 months after lyophilization or air-drying
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Inhibitor-Tolerant LAMP & RT-LAMP Mixes
Blood-Specific Mixes
PRODUCT
CAT. NO.
VOLUME REACTIONS
Superior Specificity and Quicker Time to Detection for Viral and Bacterial Targets
5 mL
800
A MDX124 | Lyo-Ready Direct DNA LAMP Blood
B MDX125 | Lyo-Ready Direct RNA/DNA LAMP Blood
Lyo-Ready Direct DNA LAMP Blood Mix
MDX124
50 mL
8,000
5 mL
800
Lyo-Ready Direct RNA/DNA LAMP Blood Mix
MDX125
50 mL
8,000
Blood is one of the most common specimens used for laboratory diagnostic testing and is useful for evaluating the function of vital organs and for diagnosing diseases such as bacterial and viral infections, cancer, cardiovascular disease, and metabolic disorders including diabetes. Bacterial cell culture is regarded as the gold standard for microbial detection from blood as molecular methods to-date have lacked the sensitivity to detect bacteria at low concentrations due to the presence of assay inhibitors among other factors. Meridian’s Lyo-Ready Direct DNA and RNA/DNA LAMP Blood Mixes are the newest solution for assays requiring high sensitivity and fast time to results and can detect DNA or RNA down to single copies in blood, serum or plasma samples. The mixes are designed to be extremely inhibitor-tolerant, enabling direct detection using crudely processed samples, making them ideal for developing next-generation, culture-free assays detecting bacterial infections such as sepsis. They outperform other commercial LAMP mixes in terms of sensitivity, specificity and speed which is demonstrated by their quicker time-to-result (TTR) and lower non-specific amplification (NTC) across a range of targets. By using innovative ready-to-use isothermal master mixes such as Lyo-Ready Direct DNA LAMP or RNA/ DNA LAMP Blood mix, assay development time for a new ultra-sensitive direct blood assay is rapid, simple and efficient. Perfect for increasing your competitive edge and capturing value faster in the rapidly growing point-of-care market.
MDX124/MDX125 | NEB WarmStart ® | Thermo SuperScript TM
(A) Lyo-Ready Direct DNA LAMP Blood ( MDX124 - red ) was lyophilized with primers for Trichomonas vaginalis , Hepatitis B Virus (HBV), Herpes Simplex Virus 2 (HSV2), Epstein-Barr Virus (EBV) or Mycoplasma pneumoniae , and (B) Lyo-Ready Direct RNA/DNA LAMP Blood ( MDX125 - red ) was lyophilized with primers for Hepatitis C Virus (HCV), HIV-1, Dengue Type 1, West Nile Virus (WNV) or Zika. The performance of the mixes was compared against NEB WarmStart ® LAMP Kit (green) and Thermo SuperScript TM IV RT-LAMP Master Mix (blue) using the same primers and DNA/RNA. Reactions were incubated at 65°C for 60 minutes. Error bars represent the standard deviation across three technical replicates. Both Lyo-Ready Direct LAMP Blood mixes delivered faster target amplification (TTR) with higher specificity, as demonstrated by the longest delay in no-template control (NTC) amplification across the range of pathogenic DNA and viral RNA targets.
Higher Specificity in the Presence of Whole Blood K2-EDTA or Plasma
A MDX124 | Lyo-Ready Direct DNA LAMP Blood
B MDX125 | Lyo-Ready Direct RNA/DNA LAMP Blood
MDX124/MDX125 | NEB WarmStart ® | Thermo SuperScript TM
(A) Lyo-Ready Direct DNA LAMP Blood ( MDX124 - red ) was lyophilized with primers for HBV, EBV, P. malaria , P. pneumoniae or HSV2, and (B) Lyo-Ready Direct RNA/DNA LAMP Blood ( MDX125 - red ) was lyophilized with primers for Dengue Type 1, HCV, West Nile Virus (WNV), Zika, or HIV-1. Their performance was tested against NEB WarmStart ® LAMP Kit (green) and Thermo SuperScript TM IV RT-LAMP Master Mix (blue). Reactions were prepared in the presence of 5-20% Blood K2-EDTA or plasma extract. The data illustrates better performance with faster amplification across the range of inhibitors for both Lyo-Ready Direct LAMP Blood mixes, with earlier TTRs across a broad range of pathogenic targets.
meridianbioscience.com/lifescience
Inhibitor-Tolerant LAMP & RT-LAMP Mixes
Saliva-Specific Mixes
High Inhibitor Tolerance and Fast Amplification to Deliver Quicker Time-to-Results (TTR) in Saliva, Sputum and Samples Containing Universal Transport Media (UTM)
PRODUCT
CAT. NO.
VOLUME REACTIONS
5 mL
800
Lyo-Ready Direct DNA LAMP Saliva Mix
MDX134
MDX134 | Lyo-Ready Direct DNA LAMP Saliva
50 mL
8,000
70 60 50 40
70 60 50 40 20 10 0 30
70 60 50 40 20 10 0 30
CMV
HSV2
ADV
5 mL
800
Lyo-Ready Direct RNA/DNA LAMP Saliva Mix
MDX135
50 mL
8,000
30
20 10 0
Saliva is ideal for use as a diagnostic specimen as it contains hormones, antibodies, growth factors, enzymes and microbes that can be used as biomarkers for disease detection. Saliva is easy-to-collect and allows non-invasive and simple sample collection/logistics, making it perfect for point of care testing. However, due to the low concentration of analytes and high concentration of inhibitors in saliva, its use in molecular diagnostics has been limited. Isothermal amplification such as LAMP is very tolerant to inhibitors and the simple workflow allows fast, sensitive, reliable results in clinical diagnosis. Meridian’s new Lyo-Ready Direct DNA and RNA/DNA LAMP Saliva Mixes are the newest advancement in molecular reagent technology and combine the benefits of a highly inhibitor-tolerant mix, with robust isothermal amplification, to enable direct detection using crudely processed samples. The mixes are uniquely designed to overcome saliva and sputum inhibitors and they do not require a DNA or RNA purification step to purify a patient sample. In addition, the 4x concentrated master mixes are formulated with lyo-excipients which are optimized for freeze-drying to create ambient-temperature stable assays. By using innovative ready-to-use isothermal master mixes such as Lyo-Ready Direct DNA LAMP or RNA/ DNA LAMP Saliva mix, assay development time for a new ultra-sensitive direct saliva assay is rapid, simple and efficient. Perfect for increasing your competitive edge and capturing value faster in the rapidly growing point-of-care market.
NTC
NTC
NTC
0% Inhibitor
30% Saliva
30% UTM + Sputum
30% VTM
0% Inhibitor
30% Saliva
30% UTM + Sputum
30% VTM
0% Inhibitor
30% Saliva
30% UTM + Sputum
30% VTM
MDX134 (Lyophilized) | NEB WarmStart ®
Primers for CMV, ADV or HSV-2 were added to Lyo-Ready Direct DNA LAMP Saliva ( MDX134 - blue ) and lyophilized, or added to NEB WarmStart ® ( green ) and kept as a liquid. The mixes were amplified using inactivated Cytomegalovirus (CMV), Adenovirus (ADV) or Herpes Simplex Virus 2 (HSV2). Reactions were prepared in the presence of 30% human saliva, 30% Universal Transport Medium (UTM) with an artificial sputum swab, and Viral Transport Medium (VTM). All reactions were incubated at 65°C for 60 minutes, and average time to results (TTR) was measured at 1:10 of end fluorescence. Error bars represent the standard deviation across four technical replicates. Results show that MDX134 (blue) has a shorter TTR than NEB WarmStart ® (green) amplification across all three sample types. The data suggests that MDX134 performs better with faster amplification across the range of inhibitors.
Superior Specificity Using Samples Containing 15% Sputum
MDX134 | Lyo-Ready Direct DNA LAMP Saliva
Amplification of Mycoplasma pneumoniae genomic DNA (200 copies) was compared using Lyo-Ready Direct DNA LAMP Saliva (MDX134) against NEB WarmStart ® in samples containing 0% or 15% artificial sputum. Reactions were incubated at 65°C for 60 minutes, and TTR was measured at 1:10 of end fluorescence. No NTCs were detected in this assay. Lyo-Ready Direct DNA LAMP Saliva ( MDX134 ) with 0% ( light blue ) and 15% ( dark blue ) artificial sputum demonstrated stronger inhibitor tolerance, retaining its fast TTR in the presence of 15% artificial sputum. In contrast, NEB WarmStart ® with 0% artificial sputum ( orange ) amplified slower, as shown by a later TTR, than either reaction using Lyo-Ready Direct DNA LAMP Saliva. In addition, in samples containing 15% artificial sputum ( red ), NEB WarmStart ® had a delayed amplification of 40 min and the longest TTR of all the reactions.
MDX134 0% Sputum | MDX134 15% Sputum NEB WarmStart ® 0% Sputum | NEB WarmStart ® 15% Sputum
Maintains Consistent Performance in Samples Containing up to 60% Saliva MDX135 | Lyo-Ready Direct RNA/DNA LAMP Saliva
Lyo-Ready Direct RNA/DNA LAMP Saliva ( MDX135 ) was lyophilized with Mycobacterium tuberculosis (MTB) primers and resuspended with increasing amounts of human saliva containing M. tuberculosis (from 10% - 72%). Reactions were incubated at 65°C for 60 minutes, and TTR was measured at 1:10 of end fluorescence. The results illustrate that Lyo-Ready Direct RNA/DNA LAMP Saliva maintains robust performance up to 60% saliva and demonstrates consistently late NTCs.
25
20
15
10
5
0
0%
10%
20%
30%
50%
60%
Saliva (%)
meridianbioscience.com/lifescience
Inhibitor-Tolerant LAMP & RT-LAMP Mixes
Stool-Specific Mixes
Significantly Higher Tolerance to Inhibitors Found in Crude Stool Material
PRODUCT
CAT. NO.
VOLUME REACTIONS
5 mL
800
MDX144 | Lyo-Ready Direct DNA LAMP Stool
Lyo-Ready Direct DNA LAMP Stool Mix
MDX144
50 mL
8,000
Adenovirus
Salmonella typhimurium
10 20 30 40 50 60
10 20 30 40 50 60
5 mL
800
Lyo-Ready Direct RNA/DNA LAMP Stool Mix
MDX145
50 mL 8,000 Diarrhoeal disease is the 8 th leading cause of death globally and the 2 nd leading cause of death in children under the age of five. It is caused by a variety of bacterial, viral and parasitic organisms and is usually spread through the fecal-oral route or by foodborne transmission. Stool has been used a diagnostic specimen to detect the presence of these organisms, and also to diagnose certain cancers and inform of overall gut health. In order to provide the right treatment and prevent serious complications, rapid identification of gastrointestinal infections is crucial. This can be carried out using point-of-care (POC) diagnostic tests that are simple to use, involve minimal-to-nosample pre-processing, do not require cold room storage and are as sensitive and specific as laboratory-based tests. Assays using stool as a specimen can pose a substantial challenge for POC assays as stool samples can be complex, containing food debris, desquamated epithelial cells and mucus, and potential LAMP inhibitors such as bile salts, polysaccharides, hematin and catabolic substances. Meridian has developed Lyo-Ready Direct DNA LAMP Stool and Lyo-Ready Direct RNA/DNA LAMP Stool Mixes to combine high inhibitor tolerance with fast, robust isothermal amplification to enable highly sensitive direct detection using crudely processed stool samples. The mixes contain excipients and an optimized buffer system that is compatible with lyophilization to create ambient-temperature stable assays which have more than two-year’s shelf-life; and are uniquely designed to overcome very high concentrations of inhibitors and so do not require a DNA or RNA purification step to purify a patient sample. Superior Assay Sensitivity in Challenging Environments such as Low Copy Input and High Bile Salt Concentration
0
0
0 mg/mL
6.6 mg/mL
0 mg/mL
6.6 mg/mL
Campylobacter jejuni
Escherichia coli O157
10 20 30 40 50 60
10 20 30 40 50 60
0
0
0 mg/mL
6.6 mg/mL
0 mg/mL
6.6 mg/mL
The impact of stool inhibitors on LAMP performance was tested for Lyo-Ready Direct DNA LAMP Stool ( MDX144 - brown ) in liquid format, NEB WarmStart ® ( green ) and Thermo SuperScript TM IV ( blue ). Mixes were prepared with primers for Salmonella typhimurium , Adenovirus, Campylobacter jejuni or E. coli O157 in the presence of 0 mg/mL or 6.6 mg/mL human stool and genomic DNA from Salmonella , Campy, E. coli or inactivated adenovirus. All reactions were incubated at 65°C for 60 minutes, and the average time to results (TTR) was measured at 1:10 of end fluorescence. Error bars represent the standard deviation across six technical replicates. Results show that MDX144 has the shortest TTR even in the presence of 6.6 mg/ mL stool, whereas NEB WarmStart ® and Thermo SuperScript TM IV have significantly delayed TTRs. The results demonstrate that MDX144 has higher tolerance to inhibitors found in stool compared to other commercial mixes. MDX144 | NEB WarmStart ® | Thermo SuperScript TM
MDX145 | Lyo-Ready Direct RNA/DNA LAMP Stool
Rotavirus
RSV virus
Zika virus
10 20 30 40 50 60
10 20 30 40 50 60
10 20 30 40 50 60
A MDX144 | Lyo-Ready Direct DNA LAMP Stool
B MDX145 | Lyo-Ready Direct RNA/DNA LAMP Stool
Salmonella typhimurium
Respiratory Syncytial Virus (mg/mL)
60
60
0
0
0
0 mg/mL
12.5 mg/mL
0 mg/mL
12.5 mg/mL
0 mg/mL
12.5 mg/mL
50
50
Norovirus
Adenovirus
10 20 30 40 50 60
10 20 30 40 50 60
40
40
30
30
20
20
10
10
0
0
0
0
0 mg/mL
12.5 mg/mL
0 mg/mL
12.5 mg/mL
1000
100
10
1
0
3
5
NTC
0
3
5
NTC
0
3
5
NTC
MDX144/MDX145 | NEB WarmStart ® | Thermo SuperScript TM
MDX145 | NEB WarmStart ® | Thermo SuperScript TM
(A) Lyo-Ready Direct DNA LAMP Stool ( MDX144 - brown ) in liquid format was used for the amplification of 1,000, 100, 10 and 1 copies of Salmonella DNA in the presence of 3 mg/mL bile salts and (B) Lyo-Ready Direct RNA/DNA LAMP Stool ( MDX145 - brown ) liquid format was used for the amplification of Respiratory Syncytial Virus (RSV) in the presence of 0 mg/mL, 3 mg/mL and 5 mg/mL bile salts. The results were compared using the same primers and DNA/RNA template for NEB WarmStart ® ( green ) and Thermo SuperScript TM IV ( blue ). Reactions were incubated at 65°C for 60 minutes, and TTR was measured at 1:10 of end fluorescence. Error bars represent the standard deviation across four technical replicates. The results demonstrate the high sensitivity of Lyo-Ready Direct DNA LAMP Stool, even at 1 copy of DNA, and both mixes’ high tolerance to bile salt.
The impact of stool inhibitors on LAMP performance was tested for Lyo-Ready Direct RNA/DNA LAMP Stool (MDX145 - brown ) in liquid format, NEB WarmStart ® ( green ) and Thermo SuperScript TM IV ( blue ). Mixes were prepared using inactivated viruses (Rotavirus, Norovirus, RSV, Zika (RNA viruses) and Adenovirus (ADV) (DNA virus)), the corresponding inactivated virus and with 0 mg/mL or 12.5 mg/mL stool. All reactions were incubated at 65°C for 60 minutes, and the average time to results (TTR) was measured at 1:10 of end fluorescence. Error bars represent the standard deviation across six technical replicates. Results show that MDX145 has a shorter TTR than NEB WarmStart ® and Thermo SuperScript TM IV in the presence of human stool. MDX145 also demonstrated better performance with faster amplification across all RNA and DNA templates in the presence and absence of human stool.
meridianbioscience.com/lifescience
Inhibitor-Tolerant LAMP & RT-LAMP Mixes
Urine-Specific Mixes
PRODUCT
CAT. NO.
VOLUME REACTIONS
Results Delivered in Half the Time with Samples Containing 20-30% Urine
5 mL
800
Lyo-Ready Direct DNA LAMP Urine Mix
MDX154
MDX155 | Lyo-Ready Direct DNA LAMP Urine
50 mL
8,000
60
Dengue Type 1 + 20% Urine
5 mL
800
Lyo-Ready Direct RNA/DNA LAMP Urine Mix
MDX155
50
50 mL
8,000
40
Urine has long been used as a diagnostic sample type for sexually transmitted infections (STIs) such as chlamydia, gonorrhoea, HSV and HPV, among many others. It has also been used to monitor hormone and metabolic disorders, renal diseases, bladder cancer, urinary tract infections (UTIs) and for monitoring recreational drug use. Urine is an ideal clinical specimen because it is excreted in large quantities, it is non-invasive, and it can be self-sampled. Lyo-Ready Direct DNA and RNA/DNA LAMP Urine Mixes are designed to deliver fast and sensitive amplification of DNA or RNA using direct detection methods. Samples do not require nucleic acid extraction and can be used directly on the assay. The mixes also contain excipients and an optimized buffer system that is compatible with lyophilization to create ambient-temperature stable assays which have more than 12 month’s shelf-life.
30
Chikungunya + 5% Urine
20
10
0
HCV + 5% Urine
WNV + 5% Urine
Dengue 1 + 20% Urine
CHIKV + 5% Urine
Zika + 20% Urine
MDX155 (Lyophilized) | NEB WarmStart ® | Thermo SuperScript TM
Results Delivered in Half the Time with Samples Containing 20-30% Urine
Lyo-Ready Direct RNA/DNA LAMP Urine ( MDX155 - yellow ) was lyophilized with primers for either Chikungunya Virus (CHIKV), Hepatitis C Virus (HCV), Zika, West Nile Virus (WNV) or Dengue Type 1. The performance of the mixes was compared against liquid formats of NEB WarmStart ® LAMP Kit ( green ) and Thermo SuperScript TM IV RT-LAMP Master Mix ( blue ) using the same primers and DNA/RNA. Reactions were prepared in the presence of 5-30% human urine. All reactions were incubated at 65°C for 60 minutes. Error bars represent the standard deviation across four technical replicates. Results show that MDX155 has earlier TTRs across a broad range of pathogenic DNA and viral RNA targets. The data illustrates Lyo- Ready Direct RNA/DNA LAMP Urine (MDX155) performs better and with faster amplification across the range of inhibitors compared to the other mixes.
MDX154 | Lyo-Ready Direct DNA LAMP Urine
60
HSV1 + 30% Urine
50
40
30
N. gonorrhoeae + 30% Urine
20
10
0
N. gonorrhoeae + 20% Urine
M. genitalium + 20% Urine
T. vaginalis + 30% Urine
HSV1 + 20% Urine
L. interrogans + 30% Urine
C. trachomatis + 30% Urine
MDX154 (Lyophilized) | NEB WarmStart ® | Thermo SuperScript TM Lyo-Ready Direct DNA LAMP Urine ( MDX154 - yellow ) was lyophilized with primers for Neisseria gonorrhoeae , Leptospira interrogans , Mycoplasma genitalium , Chlamydia trachomatis , Trichomonas vaginalis and Herpes Simplex Virus 1 (HSV1) The performance of the mixes was compared against liquid formats of NEB WarmStart ® LAMP Kit ( green ) and Thermo SuperScript TM IV RT-LAMP Master Mix ( blue ) using the same primers and DNA/RNA. Reactions were prepared in the presence of 5-30% human urine. All reactions were incubated at 65°C for 60 minutes. Error bars represent the standard deviation across four technical replicates. Results show that MDX154 has earlier TTRs across a broad range of pathogenic DNA and viral RNA targets. The data illustrates Lyo-Ready Direct DNA LAMP Urine (MDX154) performs better and with faster amplification across the range of inhibitors compared to the other mixes.
meridianbioscience.com/lifescience
Universal LAMP & RT-LAMP Mixes
PRODUCT
CAT. NO.
VOLUME REACTIONS
Superior Sensitivity with Shorter Time-to-Results (TTR) A MDX119 | Air-Dryable TM DNA LAMP Mix
5 mL
800
B MDX118 | Air-Dryable TM RNA/DNA LAMP Mix
Air-Dryable TM LAMP Mix
MDX119
50 mL
8,000
80
50
40
5 mL
800
60
Air-Dryable TM RT-LAMP Mix
MDX118
30
50 mL
8,000
40
20
5 mL
800
20
Lyo-Ready LAMP Mix
MDX097
10
50 mL
8,000
0
0
1000 copies/rxn 100 copies/rxn 20 copies/rxn
NTC
1000 copies/rxn 500 copies/rxn 100 copies/rxn 10 copies/rxn
5 mL
800
(A) Conserved regions from the S and P genes of Hepatitis B Virus (1,000, 100 and 20 copies respectively) were amplified using Air-Dryable TM DNA LAMP ( MDX119 ) in an air-dried ( red ) or liquid ( blue ) format or using NEB WarmStart ® ( green ). (B) Emesvirus (MS2) was amplified using AirDryable TM RNA/DNA LAMP ( MDX118 ) in an air-dried ( red ) or liquid ( blue ) format or using NEB WarmStart ® ( green ). Reactions were incubated at 65°C for 60 min and TTR were measured at 1:10 of end fluorescence. Error bars represent standard deviation over 4 technical replicates. At lower copy numbers, NEB WarmStart ® (green) exhibited significantly lower reaction reproducibility compared to Air-Dryable TM DNA LAMP Mix, and a lower TTR was observed for reactions using both Air-Dryable TM DNA LAMP Mix and Air-Dryable TM DNA/RNA LAMP Mix across all dilutions of the Hepatitis B gene and MS2-RNA respectively. The results therefore demonstrate greater sensitivity of Air-Dryable TM LAMP Mixes with shorter TTR across all template concentrations. MDX119/118 (Air-Dried) | MDX119/118 (Liquid) | NEB WarmStart ®
Lyo-Ready RT-LAMP 1-Step Mix
MDX108
50 mL
8,000
Meridian’s Lyo-Ready and Air-Dryable TM LAMP and RT-LAMP Master Mixes are the market-leading solution for molecular diagnostic assays requiring fast cycling, superior sensitivity, high inhibitor tolerance and ambient temperature stability. They are 4x concentrated, glycerol-free and contain specialized excipients that are compatible with lyophilization or air-drying to create ambient-temperature stable assays, or they can be used as a wet mix. They are highly inhibitor-tolerant and ultra-sensitive making them ideal for point-of-care or machine-automated multiplexing assays. As universal mixes, they are highly resistant to various qPCR inhibitors and can be used across a range of patient sample types.
PRODUCT
CAT. NO.
VOLUME REACTIONS
5 mL
800
Fast Polymerization for Quick Sample-to-Answer Time MDX097 | Lyo-Ready LAMP Mix
Lyo-Ready AOF dUTP DNA LAMP
MDX301
50 mL
8,000
20
The average time-to-result (TTR) for Lyo-Ready LAMP Mix ( MDX097 - teal green ) and NEB WarmStart ® ( light green ) were compared for three genes, 9TV10 from Trichomonas vaginalis (100 copies), hBRCA1 from human genomic DNA (200 copies) and MTB1 from Mycobacterium tuberculosis (1,000 copies). The results demonstrate the faster speed of Lyo-Ready LAMP Mix, with earlier TTR values when compared to NEB WarmStart ® . Reactions were incubated at 65°C for 60 min and TTR were measured at 1:10 of end fluorescence.
5 mL
800
Lyo-Ready AOF dUTP RNA/DNA LAMP
MDX302
50 mL 8,000 Meridian’s Lyo-Ready AOF dUTP DNA LAMP and RT-LAMP Master Mixes are also designed to prevent carryover contamination and minimize false positives, making them ideal for high-throughput and multiplex assays, and the BSA-free formulation reduces reliance on animal-sourced reagents while enhancing efficiency and consistency. Faster Time-to-Result (TTR) and Better Reproducibility A MDX301 | Lyo-Ready AOF dUTP DNA LAMP B MDX302 | Lyo-Ready AOF dUTP RNA/DNA LAMP
15
10
5
0
9TV10 - T. Vaginalis
hBRCA-1M - Human DNA MTB1 - M. tuberculosis
MDX097 | NEB WarmStart ®
30
30
Fast Polymerization for Quick Sample-to-Answer Time MDX118 | Air-Dryable TM RNA/DNA LAMP Mix
25
25
20
20
Air-Dryable TM RNA/DNA LAMP ( MDX118 - teal green ) was compared to NEB WarmStart ® ( light green ) for detection of EBV DNA (1,000 ipc) and MS2 RNA (1,000 ipc). Reactions were incubated at 65°C for 60 min and TTR were measured at 1:10 of end fluorescence. Amplification plots of 3 technical replicates demon- strate AirDryable TM RNA/DNA LAMP Mix has earlier TTR and no non-specific amplification compared to NEB WarmStart ® , which has detectable amplification for non-template controls (NTC).
15
15
10
10
5
5
0
0
BRCA1
M. pneumoniae
T. vaginalis
Hepatitis C
West Nile Virus
Chikungunya
(A) Lyo-Ready AOF dUTP DNA LAMP ( MDX301 - teal green ) was amplified with BRCA1 (1,000 copies), M. pneumoniae (100 copies) and T. vaginalis (100 copies), and (B) Lyo-Ready AOF dUTP RNA/DNA LAMP ( MDX302 - teal green ) was amplified with Hepatitis C Virus (100 copies), West Nile Virus (100 copies) and Chikungunya (1,000 copies). They were compared to the same samples amplified with NEB WarmStart ® Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG) ( light green ). The results demonstrate the faster speed (time to results) and better reproducibility (shorter error bars) of the Lyo-Ready AOF dUTP LAMP mixes compared to other suppliers.
MDX118 | NEB WarmStart ®
meridianbioscience.com/lifescience
APRIL | 2026
LYO-READY & AIR-DRYABLE TM LAMP & RT-LAMP MASTER MIXES
Performance is Not Impacted by Lyophilization or Subsequent Room-Temperature Storage
MDX301 | Lyo-Ready AOF dUTP DNA LAMP BRCA1
M. pneumoniae
T. vaginalis
Liquid | Lyophilized
Freshly prepared Lyo-Ready AOF dUTP DNA LAMP ( MDX301 ) as a liquid mix ( blue ) was compared to lyophilized mix ( red ) that had been incubated for 1 year at ambient temperature, amplifying BRCA1, M. pneumoniae or T. vaginalis . The results show equivalent performance between fresh liquid mix and lyophilized mix demonstrating high stability and reproducibility of the mixes.
PRODUCT LIST
MDX124 | Lyo-Ready Direct DNA LAMP Blood MDX125 | Lyo-Ready Direct RNA/DNA LAMP Blood Blood MDX144 | Lyo-Ready Direct DNA LAMP Stool MDX145 | Lyo-Ready Direct RNA/DNA LAMP Stool Stool
MDX134 | Lyo-Ready Direct DNA LAMP Saliva MDX135 | Lyo-Ready Direct RNA/DNA LAMP Saliva Saliva MDX154 | Lyo-Ready Direct DNA LAMP Urine MDX155 | Lyo-Ready Direct RNA/DNA LAMP Urine Urine
Universal
MDX097 | Lyo-Ready DNA LAMP MDX119 | Air-Dryable TM DNA LAMP
MDX108 | Lyo-Ready RNA/DNA LAMP MDX118 | Air-Dryable TM RNA/DNA LAMP
MDX301 | Lyo-Ready AOF dUTP DNA LAMP
MDX302 | Lyo-Ready AOF dUTP RNA/DNA LAMP
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